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| Detect caspases by Western blotting using anti-caspase antibodies ICT is offering a new line of rabbit polyclonal antibodies to detect caspases for Western blotting. ICT’s antibodies were tested for cross-reactivity with caspases 1-10 and 14, and do not cross-react, unless stated. (Please contact us for pricing.) |
| • Anti-Caspase-1 recognizes human proenzyme (45 kDa) and large (22 kDa) subunit of the mature
enzyme. • Anti-Caspase-2 recognizes human proenzyme (49 kDa) and the large (19 kDa) and small (12 kDa) subunits of the mature enzyme. • Anti-Caspase-3 recognizes human proenzyme (32 kDa) and the large (17 kDa) and small (12 kDa) subunits of the mature enzyme. • Anti-Caspase-4 recognizes human proenzyme (43 kDa) and large (19 kDa) and small (10 kDa) subunits of the mature enzyme. |
• Anti-Caspase-5
recognizes human proenzyme (48
kDa) and large (22 kDa) and small (10 kDa) subunits of the mature enzyme; slight cross-reactivity with caspase
4. • Anti-Caspase-7 recognizes human proenzyme (34 kDa) and large (20 kDa) subunit of the mature enzyme. • Anti-Caspase-8 recognizes human proenzyme (55 kDa) and small (11 kDa) subunit of the mature enzyme. • Anti-Caspase-9 recognizes human proenzyme (46 kDa) and large (20 kDa) and small (10 kDa) subunits of the mature enzyme. |
• Anti-Caspase-11 recognizes mouse proenzyme (43
kDa) and large (23 kDa) and small (10 kDa) subunits of the mature enzyme. No cross-reactivity with human caspases 1-10 or 14, nor with mouse caspases 1 and
3. • Anti-Caspase-14 recognizes human proenzyme (28 kDa) and large (17 kDa) and small (10 kDa) subunits of the mature enzyme. |
| Do ELISAs or immunoblotting with cathepsin antibodies ICT is offering a new line of rabbit polyclonal antibodies to detect human cathepsins for Western blotting. ICT’s antibodies were tested for cross-reactivity with and do not cross-react, unless stated. (Please contact us for pricing.) |
| • Anti-Cathepsin B was generated using cathepsin B purified from human liver as the immunogen. It does not cross-react with cathepsin D, H, or L. Use in an ELISA at 1:500 or for immunprecipitation at 1:4000 with colorimetric substrates. • Anti-Cathepsin D was generated using recombinant cathepsin D as the immunogen. It recognizes latent and active cathepsin D (from 45 kDa to 27 kDa) and does not cross-react with cathepsin B, H, or L. Recommended for immunoblotting at 1:2000 for colorimetric substrates, and 1:6000 for chemiluminescent substrates. |
• Anti-Cathepsin G was generated using cathepsin G purified from human neutrophils as the
immunogen. It does not cross-react with cathepsin B, D, H, or L, nor with granzyme B. Use in an ELISA at 1:1000 or for
immunprecipitation at 1:4000 with colorimetric substrates. • Anti-Cathepsin H was generated using cathepsin H purified from human liver as the immunogen. It does not cross-react with cathepsin B, D, or L. Use in an ELISA at 1:500 or for immunprecipitation at 1:4000 with colorimetric substrates. |
• Anti-Cathepsin L was generated using cathepsin L purified from human liver as the immunogen. It does not cross-react with cathepsin B, D, or H. Use in an ELISA at 1:250 or for immunprecipitation at 1:8000 with colorimetric substrates. |
| Do kinetic studies with purified proteases ICT is offering a new line of highly active caspase enzymes for high-throughput screening, cleavage studies, and enzyme kinetics, among other applications. Most are >90% pure by SDS-PAGE. 1 U = 1 pmol/min at 30C using the substrates listed. |
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ICT is offering a new line of active cathepsin enzymes and related proteases purified from human liver or neutrophils. Use them for kinetic studies, to cleave target substrates, or to screen for inhibitors.
(Please contact us for pricing.)
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| Copyright
2006 updated 02/06/2006 |
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