APOPTOSIS CASPASE DETECTION KITS FOR IN VITRO (FLICA) AND IN VIVO USE (FLIVO) AND IN REAL TIME (MAGIC RED)

Caspase Detection Kits
FLICA™: Quantitate all caspase activity (and apoptosis) in whole living cells
                 Quantitate specific caspase 1, 2, 3&7, 6, 8, 9, 10, or 13 with individual FLICA™ kits
FLIVO™: Visualize caspase activity in tissues in live animals
Magic Red™: Watch caspase activity progress in real time

FLICA™: Quantitate caspase activity (and apoptosis) in whole living cells in vitro  

Green Fluorescence Kits:
Poly Caspases (all) 
Caspase 1 
Caspase 2 
Caspases 3&7 
Caspase 6 
Caspase 8 
Caspase 9 
Caspase 10 
Caspase 13 

Red Fluorescence Kits:
Poly Caspases (all) 
Caspases 3&7 
Caspase 9

References

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FLICA™ is cell-permeant so you don't have to lyse the cells or permeabilize the membranes. Once added to the culture media, FLICA™ passes through the cell wall. If there is an active caspase enzyme inside the cell, it will form a covalent bond with FLICA™ and retain the fluorescent signal within the cell.  Because the caspase enzyme itself binds with FLICA™, there is no interference from pro-caspases nor inactive forms of the enzyme.  FLICA™ makes it very easy to identify caspase-positive cells. 

4 out of 5 cells are apoptotic: Jurkat cells were labeled with ICT's Poly-Caspases FLICA™ kit (cat.# 92). 4 cells fluoresce green (left), while the grey image (right) reveals 5 cells in the field. The 4 green cells are apoptotic = 80% of cells in this experiment had active caspases. The level of fluorescence can be quantified on a fluorescence plate reader or flow cytometer. Data courtesy of Dr. Brian W. Lee, ICT.

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FLIVO™: Detect caspase-positive tissues in live animals in vivo

Available in green and red fluorescence.

apoptosis before treatment

apoptosis after treatment

This drug is working to treat FSaII fibrosarcoma tumors.
The level of apoptotic cells significantly increased after treatment (there is an increased level of green fluorescence, below). Data courtesy of Dr. R. Griffin, (U of MN, U of AR). 

Read the details of this experiment (pdf)

     FLIVO™ reagents are based cell-permeant inhibitors specifically targeted by all active caspases: they are poly-caspase probes that have been optimized for injection into live animals. 
     After injection IV, the fluorescent probe (red or green) enters the cell and forms a covalent bond with active caspases; any unbound reagent is washed out of the cell by the natural circulation of the animal. Cells with active caspases fluoresce, non-apoptotic cells do not. No additional processing steps are necessary after sacrifice; simply prepare tissue samples as usual.
     FLIVO™ has successfully labeled tumors, splenocytes, bone marrow, brain, and eye tissues. Use it to assess the efficacy of chemotherapy (did the treatment induce apoptosis?) or monitor neurodegeneration (is the brain dying?). Available in green and red fluorescence. FLIVO™ makes it easy to label apoptosis within the animal. Learn a lot more about FLIVO™

References

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Magic Red™: Watch caspase activity progress over time

Real-time MR kits can detect:
Caspases 3&7 (apoptosis) 
Cathepsin B 
Cathepsin K 
Cathepsin L 

ICT's MR Caspases 3&7 kit clearly distinguishes apoptotic from non-apoptotic cells. The top 3 THP-1 cells have active caspases (they fluoresce red) the bottom 3 cells do not (they do not fluoresce). Read this publication

     ICT's Magic Red™ kits measure apoptosis via active caspases 3&7 in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. These unique kits are not ELISAs and do not use antibodies - instead they are based on cell-permeant substrate reagents specifically targeted by active caspases 3 & 7 enzymes. 
     Just culture your cells, add MR to the media, and incubate. If the enzymes are active, they will cleave the reagent causing it to fluoresce red. MR kits are true substrate assays - the red fluorescent label is quenched until it is actually cleaved by active enzymes. Only cells with active enzymes will fluoresce, so you don’t get any signal from pro-enzymes or inactive forms of the enzyme. Watch 2 movies of real-time caspase activity, courtesy of Dr. M. Purschke, MA General Hospital: movie clip of cells ; movie of fluorescence. ICT’s MR kits give a clear picture of apoptotic versus non-apoptotic cells and enable you to monitor caspase activity over time. Learn a lot more about Magic Red™

References

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Want to detect non-caspase-mediated apoptosis? Use MitoPT™

Need a positive control? Use ICT's staurosporine or other inducing reagent (accessories).