Quantitate cholinesterase and caspase activity in whole living cells

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     With ICT’s new cholinesterase assay (Ph-Fl, patent pending), researchers now have an easy way to directly quantitate and localize active cholinesterase enzymes in live, intact cells - no lysis or permeabilization steps are required (Figure 1). 
     This unique kit is not an ELISA and does not use antibodies - instead it is based on physostigmine, a known cholinesterase inhibitor, linked to a green fluorescent label, fluorescein (Fl). ICT’s Ph-Fl reagent is cell permeant, so you don’t have to lyse the cells or permeabilize the membranes. Because this kit does not use antibodies, there is no cross-reaction with inactive or pro forms of the enzyme. Only cells with the active cholinesterase enzyme will fluoresce. 
     Just culture your cells, add the cholinesterase reagent to the media, incubate, and wash the cells. If active cholinesterase enzymes are present, they will bind to Ph-Fl, and retain it on or within the cell, generating a green fluorescent signal. 
     Use the cholinesterase kit with ICT's SR FLICA kits to concurrently detect apoptosis.  Just add this reagent to the cells at the same time you add Ph-Fl. If active caspases are present in the cells, they will fluoresce red, clearly 
distinguishing them from non-apoptotic cells (Figure 1). 

For more information, please:
Read our latest paper published in Cell Cycle 4:1, 140-147; Jan 2005!
Read our Cholinesterase manual.
Read our red SR FLICA^TM manual .
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