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Jurkat cells were stained with ICT’s MitoPT™-JC1 dye.
Healthy cells fluoresce red, dying
cells fluoresce green.
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1. MitoPT™-JC1 green and red fluorescence
2. MitoPT™-TMRE red fluorescence
3. MitoPT™-TMRM
red fluorescence
• Easy
Just add directly to the media; healthy cells will fluoresce orange/red.
• Fast Typical incubation is 15-20
minutes.
• Accurate Orange/red signal is reduced in dying
cells.
• Sensitive Easily distinguish positive from negative
populations.
• Quantitative Analyze with a flow cytometer, fluorescence plate reader, or fluorescence
microscope.
• Whole cell analysis The reagents
are cell-permeant so you do not have to lyse the cells.
Order
now! 1-800-829-3194.
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Healthy
cells fluoresce red, dying
cells fluoresce green. Jurkat cells were stained with ICT’s MitoPT™-JC1 dye and analyzed with a fluorescence microscope containing a long band path filter (excitation at 490 nm and emission >510 nm). In non-apoptotic healthy cells (2 cells
on top), the reagent aggregates inside intact mitochondria and fluoresces red. As the mitochondrial membrane potential drops and cells enter apoptosis, the dye disperses throughout the cell. The reagent then assumes a monomeric form and fluoresces green (3 cells
on bottom). |
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MitoPT™-JC1 Kits
(green and red) |
| #
tests |
cat# |
price |
| 25 |
924 |
$169 |
| 100 |
911 |
$329 |
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ICT’s original MitoPT™-JC1 Mitochondrial Permeability Transition kit can
be used to quantitate apoptosis and mitochondrial functionality
- non-apoptotic cells with healthy mitochondria appear red, apoptotic cells appear green.
ICT’s unique MitoPT™-JC1 reagent
is based on a fluorescent cationic dye, commonly known as JC-1. Just culture your cells and add MitoPT™-JC1
to the media. MitoPT™-JC1 easily penetrates cells and healthy mitochondria, where it aggregates and fluoresces red. As the mitochondrial membrane potential
collapses, MitoPT™-JC1 is diffused throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This
can be read with a fluorescence plate reader, flow cytometer, or
fluorescence microscope.
MitoPT™-JC1 excites at 488nm, and emits at
>590nm. Read
cells with a fluorescence microscope, plate reader, or flow cytometer.
Sample
protocol for suspension cells (read manual):
1.
Culture your cells individually or up to 1 x
106 cells/mL.
2. Induce apoptosis following your protocol.
3. Reconstitute the reagent with
125uL or 250uL DMSO to form the stock concentrate (which can be frozen for future use).
4. Dilute the stock concentrate with
the 1X Assay Buffer to form the working solution.
5. Add ~10uL of the
working solution directly to a 1mL aliquot of your cell culture for labeling.
6. Incubate 15
minutes -1 hour.
7. Wash and spin cells
twice.
8. Analyze data using a
fluorescence microscope, plate reader, or flow
cytometer.
 |
MitoPT-JC1
kit, catalog. #911
Kit contains: MitoPT™-JC1
reagent, and assay buffer.
|
Order
now! 1-800-829-3194.
|
MitoPT™-JC1
is
easy
fast
reliable
sensitive
quantitative
Try
it today
1-800-829-3194
Order
|
|
MitoPT™-TMRE
kits contain: MitoPT™-TMRE reagent,
and assay buffer.
|
MitoPT™-TMRE Kits
(red) |
| #
tests |
cat# |
price |
| 500 |
925 |
$349 |
|

Order
now! 1-800-829-3194.
|
MitoPT-TMRE
is
easy
fast
reliable
sensitive.
Try
it today
1-800-829-3194
Order
|
|
MitoPT™-TMRM
kits contain: MitoPT™-TMRM reagent,
and assay buffer.
|
MitoPT™-TMRM Kits
(red) |
| #
tests |
cat# |
price |
| 500 |
926 |
$349 |
|
Order
now! 1-800-829-3194.
|
MitoPT-TMRM
is
easy
fast
reliable
sensitive.
Try
it today
1-800-829-3194
Order
|