MITOCHONDRIAL MEMBRANE POTENTIAL CYTOCHROME C RELEASE APOPTOSIS DETECTION KITS FOR IN VITRO USE JC-1 JC1 TMRE TMRM

MitoPT Mitochondrial Membrane Permeability Transition Kits
Changes in the mitochondrial membrane potential can correlate with cytochrome c release and the initiation of apoptosis. ICT now has 3 kits to easily assess changes in delta¥m

Jurkat cells were stained with ICT’s MitoPT™-JC1 dye. Healthy cells fluoresce red, dying cells fluoresce green.

     1. MitoPT™-JC1 green and red fluorescence
     2. MitoPT™-TMRE red fluorescence
     3. MitoPT™-TMRM red fluorescence 

• Easy Just add directly to the media; healthy cells will fluoresce orange/red. 
• Fast Typical incubation is 15-20 minutes.
• Accurate Orange/red signal is reduced in dying cells.
• Sensitive Easily distinguish positive from negative populations.
• Quantitative Analyze with a flow cytometer, fluorescence plate reader, or fluorescence microscope.
• Whole cell analysis The reagents are cell-permeant so you do not have to lyse the cells. 

Order now!  1-800-829-3194.

Green & Red MitoPT™-JC1

Healthy cells fluoresce red, dying cells fluoresce green. Jurkat cells were stained with ICT’s MitoPT™-JC1 dye and analyzed with a fluorescence microscope containing a long band path filter (excitation at 490 nm and emission >510 nm). In non-apoptotic healthy cells (2 cells on top), the reagent aggregates inside intact mitochondria and fluoresces red. As the mitochondrial membrane potential drops and cells enter apoptosis, the dye disperses throughout the cell. The reagent then assumes a monomeric form and fluoresces green (3 cells on bottom).

      ICT’s original MitoPT™-JC1 Mitochondrial Permeability Transition kit can be used to quantitate apoptosis and mitochondrial functionality - non-apoptotic cells with healthy mitochondria appear red, apoptotic cells appear green.  
     ICT’s unique MitoPT™-JC1 reagent is based on a fluorescent cationic dye, commonly known as JC-1. Just culture your cells and add MitoPT™-JC1 to the media. MitoPT™-JC1 easily penetrates cells and healthy mitochondria, where it aggregates and fluoresces red. As the mitochondrial membrane potential collapses, MitoPT™-JC1 is diffused throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This can be read with a fluorescence plate reader, flow cytometer, or fluorescence microscope. 
    MitoPT™-JC1 excites at 488nm, and emits at >590nm. Read cells with a fluorescence microscope, plate reader, or flow cytometer.

Sample protocol for suspension cells (read manual):

1. Culture your cells individually or up to 1 x 10⁶ cells/mL. 
2. Induce apoptosis following your protocol. 
3. Reconstitute the reagent with 125uL or 250uL DMSO to form the stock concentrate (which can be frozen for future use). 
4. Dilute the stock concentrate with the 1X Assay Buffer to form the working solution. 
5. Add ~10uL of the working solution directly to a 1mL aliquot of your cell culture for labeling. 
6. Incubate 15 minutes -1 hour. 
7. Wash and spin cells twice.
8. Analyze data using a fluorescence microscope, plate reader, or flow cytometer.

MitoPT-JC1  kit, catalog. #911 Kit contains: MitoPT™-JC1 reagent, and assay buffer.

Order now!  1-800-829-3194.

MitoPT™-JC1 is
easy
fast
reliable
sensitive
quantitative

Try it today
1-800-829-3194
Order

Red MitoPT™-TMRE

 MitoPT™-TMRE kits contain: MitoPT™-TMRE reagent, and assay buffer.

Order now!  1-800-829-3194.

MitoPT-TMRE is
easy
fast
reliable
sensitive.

Try it today
1-800-829-3194
Order

Red MitoPT™-TMRM

 MitoPT™-TMRM kits contain: MitoPT™-TMRM reagent, and assay buffer.

Order now!  1-800-829-3194.

MitoPT-TMRM is
easy
fast
reliable
sensitive.

Try it today
1-800-829-3194
Order

Read all references (pdf).

Please contact Sally Hed with questions 952-888-8788 x10 sally@immunochemistry.com .