 |
|
|
|
Improve your ELISA using PB1
PB1
is
a well-tested
formulation of buffers and salts
designed to effectively balance the pH without disrupting
antibody-sandwich ELISAs and antigen-down
ELISA
binding reactions. Also use it as a base to
create your own ELISA buffers or for other applications
in the lab like dialyzing proteins and running samples
over HPLC and Protein G columns.
prices
& sizes
ICT’s 10X Phosphate Buffered Saline (PBS or PB1)
is a well-tested
formulation of buffers and salts
designed to effectively balance the pH without disrupting ELISA
binding reactions.
Also use it as a base to
create your own ELISA buffers or for other
applications in the lab like dialyzing proteins
and running samples over HPLC and Protein G
columns.
ICT's
PB1 is supplied as a 10x concentrate
to conserve valuable storage space. Simply add 9
parts deionized water to 1 part PBS, mix,
and it’s ready to use. No pH adjustment is
required.
After
dilution, 1x PBS will suppress bacterial
growth for up to 6 months at room temperature and
12 months at 2°-8°C. The concentrated buffer
will suppress bacterial growth for at least 12
months at room temperature and 18
months at 2°-8°C.
|
|
How
do I use PBS?
Simply dilute
PB1 1:10 (for
example, add 100mL PB1 to 900mL diH20
for a final volume of 1000mL), let the solution stir for 15
minutes. As
PB1 is concentrated, crystalline
precipitates may form in the bottle, especially when
refrigerated. If this happens,
gently warm or mix the buffer until all crystals are dissolved.
Do not let it boil.
Help!
I can't get my assay to work!
Just
give use a call at 1-800-829-3194. To
help you develop your assay, ICT has provided some
background information on ELISA technology (see What
is an Immunoassay , What's
in a test and what is an ELISA protocol? ),
some calculation worksheets (see How
Do I Coat Plates ), and offers consultation
services in assay development (see Immunoassay
Development ). |
| 10x
PBS |
|
Size |
Catalog
# |
$
Price USD |
| 25
mL |
6252 |
$25
|
| 100
mL |
6157 |
$36
|
| 500
mL |
6158 |
$109
|
| 1
L |
6159 |
$198
|
| 10
L |
6160 |
$1,782
|
Order today!
Just call
1-800-829-3194
or download our simple order
sheet (Word) or PDF
main: 952-888-8788
fax: 952-888-8988
|
|
|
 |
|
Free
with every order!
Designed as a 96-well microtiter
plate, these cool 4x6" sticky-notes will help keep your experiments
organized.
To order refills, call us at 1-800-829-3194.
| |
| |
 |
| PB1
is a great base! ICT's
PBS can help you:
-
Run
cleaner HPLC and Protein G columns. ICT's
PBS is meticulously manufactured with the high
quality ingredients to reduce contaminants.
-
Create
your own ELISA buffers. Use PB1 as a base
for your own buffer formulations.
-
Decrease
technician time to reduce costs.
By using ICT's ready-to-use reagents, you don't have
to waste time preparing buffers.
-
Improve
precision and reduce plate-to-plate variability.
Using ICT's stabilizing diluents, plates may be
prepared in batches to be used over time, which
increases consistency and provides improved
plate-to-plate precision over extensive storage
periods.
-
Increase
assay reproducibility.
By using consistent reagents from a reliable source,
your ELISAs may become more reproducible.
-
Enhance
signal-to-noise ratios for improved assay
sensitivity.
ICT's ELISA reagents provide efficient plate coating
and blocking, sample and conjugate dilution, and
plate washing between incubation steps, which
promote a strong signal while minimizing the
nonspecific signal (noise), thus improving assay
sensitivity.
|
|
|
Order today!
Just call
1-800-829-3194
or download our simple order
sheet (Word) or PDF
main: 952-888-8788
fax: 952-888-8988
|
|
