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| Dilute
your standards and samples better.
A sample diluent is a buffer in which to dilute
serum or antigen samples so they read within the
functional range of the assay. ICT
has created 3 proprietary sample diluent formulations
for use with antigen-down and sandwich ELISAs.
General
Purpose Sample Diluent (SD1)
Use
this diluent for:
Serum
Ascites
Cell culture
supernatant samples
Plasma
Sample Diluent (Antigen-Down) (SD2)
Use
this diluent for:
Antigen-down testing of plasma samples
Inhibition
of complement and thrombin activity (clotting)
Neptune
Sample Diluent (SD3)
Use this diluent for:
Samples with high nonspecific
binding
Improved
antibody-antigen interaction
Optimization
Pack (SDOP),
includes 1 bottle of each sample diluent (100 mL each).
We can give you a good idea of which sample diluent to
try, but sometimes, it's just best to try each one in
your assay system with your samples.
prices
& sizes
What
is a sample diluent?
ICT’s
unique serum sample diluents are specifically
formulated for dilution of animal and bird serum
samples in antigen-down ELISA formats. Sample
diluents are utilized to dilute serum or antigen
samples into the functional range of the
assay.
Due to the finite binding
capacity of the coated ELISA plate surface,
certain high samples may overload this capacity,
thus requiring dilution prior to testing in the
assay. Sample diluents can also reduce background
noise associated with nonspecific bridging of
signal-generating conjugates to the plate
surface.
Hyperimmunized serum
samples contain nonspecific IgG antibody that may
interfere with the titration signal. Proprietary
additives have been incorporated into these
diluents that minimize nonspecific binding
interactions of the nonspecific IgG in the
samples, thus decreasing background noise.
These sample diluents can
be used to titrate rabbit, mouse, goat, and
chicken serum samples, along with monoclonal cell
culture supernatants. Antigen samples may
also be diluted with sample diluents for
evaluation in sandwich ELISA formats.
How
can a sample diluent improve my assay?
By
accurately reading and quantitating high-titer
samples. High-titer samples may overload the
finite binding capacity of the coated ELISA plate
surface, thus requiring dilution prior to testing
in the assay.
How
will diluting the samples affect my calculations?
Simply
multiply the value determined from the curve by your dilution factor. For
example, if you diluted your sample 1:2 (250uL sample into 250uL sample diluent)
and this yielded an OD of 0.437, when read off the curve, the OD of 0.437
correlated to 200 pg/mL, you would simply multiply 200pg/mL by 2 (your dilution
factor) yielding a value of 400pg/mL, meaning that the original starting sample
had 400pg/mL of analyte present.
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General
Purpose Sample Diluent (SD1)
SD1
is formulated for diluting goat, rabbit, mouse,
and human sera, as well as cell culture media and
mouse ascites fluid. SD1 can also be used
for dilution of mammalian and chicken serum
samples in antigen-down ELISA formats.
Hyperimmunized serum samples contain nonspecific
IgG antibody that may interfere with the
titration. Proprietary additives have been
incorporated into this diluent that minimize
nonspecific binding interactions of the non
specific IgG in the samples, thus decreasing
background noise. Dilutions of the antigen
standard for use in sandwich ELISA formats have
also been carried out using this diluent.
SD1
contains a buffered BSA protein base to
assure a constant pH and solute environment
favorable to antibody-antigen interactions.
It also contains an antimicrobial agent to provide
a solution free of bacterial contamination.
Plasma
Sample Diluent (Antigen-Down) (SD2)
SD2
is formulated specifically for use with plasma
samples being tested in an antigen-down ELISA
format. This diluent contains proprietary
additives in a buffered BSA protein base
with which provide an assay environment that
minimizes nonspecific binding while maintaining
proper ionic strength and pH for efficient
antibody-antigen interaction. Additional
additives have been included to inhibit thrombin
(clotting) and complement activity during the
plasma sample incubation period. SD2 also
contains an antimicrobial agent to provide a
solution free of bacterial contamination.
Neptune
Sample Diluent (SD3)
This
diluent contains proprietary additives in a
buffered PBS base with which provide an
assay environment that minimizes nonspecific
binding while maintaining proper ionic strength
and pH for efficient antibody-antigen
interaction. SD3 does not contain any
mammalian proteins. SD3 also contains an
antimicrobial agent to provide a solution free of
bacterial contamination.
Optimization
Pack - save 15%!
To create
the best assay possible, sometimes it's best to
just try each sample diluent and see which one
yields the highest specific signal with your
sample type. Buy the optimization pack to receive
100mL of each sample diluent and save 15%!
The
optimization pack (catalog #959) includes one
100mL bottle of each: catalog #647 SD1, #694 SD2,
and #6124 SD3.
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| Sample
Diluents |
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General Purpose
Serum Diluent (SD1) |
Size |
Catalog
# |
$
Price USD |
| 100
mL |
647 |
$45
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| 500
mL |
648 |
$139
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| 1
L |
649 |
$240
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| 10
L |
675 |
$2,160
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Plasma Sample
Diluent (Antigen-Down) (SD2) |
Size |
Catalog
# |
$
Price USD |
| 100
mL |
694 |
$58
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| 500
mL |
695 |
$178
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| 1
L |
696 |
$324
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| 10
L |
697 |
$2,916
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Neptune Sample
Diluent (SD3) |
Size |
Catalog
# |
$
Price USD |
| 100
mL |
6124 |
$49
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| 500
mL |
6125 |
$149
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| 1
L |
6126 |
$261
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| 10
L |
6127 |
$2,349
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Optimization Pack of all
3 Sample Diluents (SOTP) |
100
mL each |
959 |
$129 |
Order today!
Just call
1-800-829-3194
or download our simple order
sheet (Word) or PDF
main: 952-888-8788
fax: 952-888-8988
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Free
with every order!
Designed as a 96-well microtiter
plate, these cool 4x6" sticky-notes will help keep your experiments
organized.
To order refills, call us at 1-800-829-3194.
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| Dilute
your samples better! ICT's
sample diluents can help you:
-
Conserve
valuable reagents.
Use of ICT's coating and blocking buffers promotes a
higher specific signal, therefore less of your
antigen or antibody may be needed in the plate
coating process. In addition, a lower
concentration of detection molecules may be needed
to generate a signal.
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Decrease
technician time to reduce costs.
By using ICT's ready-to-use reagents, you don't have
to waste time preparing buffers.
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Increase
the shelf-life of your plates.
Depending on the activity of the coated protein,
plates may be stored at RT or 2°-8°C for several
months, or even years under proper conditions when
using ICT's diluents.
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Increase
efficiency by preparing plates in large batches.
Using
ICT's coating and blocking reagents, plates may be
prepared in large batches and remain stable over
time.
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Improve
precision and reduce plate-to-plate variability.
Using ICT's stabilizing diluents, plates may be
prepared in batches to be used over time, which
increases consistency and provides improved
plate-to-plate precision over extensive storage
periods.
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Increase
assay reproducibility.
By using consistent reagents from a reliable source,
your ELISAs may become more reproducible.
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Enhance
signal-to-noise ratios for improved assay
sensitivity.
ICT's ELISA reagents provide efficient plate coating
and blocking, sample and conjugate dilution, and
plate washing between incubation steps, which
promote a strong signal while minimizing the
nonspecific signal (noise), thus improving assay
sensitivity.
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Minimize
sample matrix effects. Use
of ICT's sample and assay diluents can reduce errors
induced from the sample solution that may result in
under-estimation of the concentration of the analyte
in biological samples.
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Order today!
Just call
1-800-829-3194
or download our simple order
sheet (Word) or PDF
main: 952-888-8788
fax: 952-888-8988
Help!
I can't get my assay to work!
Just
give use a call at 1-800-829-3194. To
help you develop your assay, ICT has provided some
background information on ELISA technology (see What
is an Immunoassay , What's
in a test and what is an ELISA protocol? ),
some calculation worksheets (see How
Do I Coat Plates ), and offers consultation
services in assay development (see Immunoassay
Development ).
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