ICT’s ELISA Wash Buffer formulation is an
optimal formulation of pH stabilizers, salts, and
detergents designed to effectively remove excess
material from the microtiter plate wells without
disrupting the ELISA binding reaction. By
maintaining the proper buffering environment,
unbound components can be washed away without
suppressing antigen-antibody binding interactions,
thereby reducing nonspecific background noise and
increasing the specific signal. ICT’s Wash
Buffer is compatible with all routinely used
conjugate components such as HRP, AP, and avidin,
among others.
ICT's
ELISA Wash Buffer is supplied as a 10x concentrate
to conserve valuable storage space. Simply add 9
parts deionized water to 1 part ELISA Wash, mix,
and it’s ready to use. No pH adjustment is
required. ELISA Wash contains a non-azide
non-mercury preservative that will not interfere
with antibody-antigen binding interactions. After
dilution, ELISA Wash will suppress bacterial
growth for up to 6 months at room temperature and
12 months at 2°-8°C. The concentrated buffer
will suppress bacterial growth for at least 18
months at 2°-8°C.
How
much WB1 do I need?
Each wash will use about 30-40mL per plate. If
you are washing 3 times between steps of your ELISA, you will use about 120mL
of 1x wash buffer per wash cycle. With 2 wash cycles, that's about 240mL
per ELISA.
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How
do I use WB1?
Simply dilute
WB1 1:10 (for
example, add 100mL WB1 to 900mL diH20
for a final volume of 1000mL), let the solution stir for 15 minutes, and
wash your plate. As
WB1 is concentrated, crystalline
precipitates may form in the bottle, especially when
refrigerated. If this happens,
gently warm or mix the buffer until all crystals are dissolved.
Do not let it boil.
How
do I wash plates using WB1?
To
wash microtiter plates, the diluted wash buffer
may be dispensed through a squirt bottle, a plate
washer, through a multi-channel pipette, or
through an automated system. If washing by
hand, be sure to fill the wells all the way up
with 1x wash buffer (about 400uL/well). Then
aspirate or dump out the buffer and repeat for a
total of 2-4 washes. After the final dump or
aspiration, pound the plate on paper towels to
remove any excess liquid. We do not
recommend submerging the entire plates in a bath
as many of the wells will become contaminated,
which decreases the reliability of the assay.
If you are washing the
plate during the coating process, we recommend 2-3
washes between steps. If you are washing the
plate as part of an ELISA procedure, we recommend
2-4 washes between steps.
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