Measuring Caspase-1 Activity
Caspase-1 (ICE)
• Originally identified as Interleukin -1β Converting Enzyme or ICE
• Cleaves IL-1 beta and IL-18 into active forms
• Creation of the inflammasome during host responses to pathogens leads to the activation of caspase-1
• Active caspase-1 plays an antimicrobial role during infection, leading to pyroptosis
• Inflammation-related disease models have illustrated a role for caspase-1 in asthma, rheumatoid arthritis, multiple sclerosis and other disorders
Activated THP-1 cells show caspase-1 (ICE) activity with the green fluorescent FAM-FLICA Caspase-1 Detection Assay
FAM-FLICA™ Caspase-1 Assay
This in vitro assay employs a fluorescent green inhibitor probe to detect active caspase-1 enzymes in living cells or tissue samples. 
View assay details and data: http://www.immunochemistry.com/products/green-flica-caspase-1-detection-assay-kit.html
Recent Citations
E. D. Murphey. Cecal Ligation and Puncture-Induced Impairment of Innate Immune Function Does Not Occur in the Absence of Caspase-1. J. Immunol. 2011; 187:905-910.
Mélanie Anne Hamon and Pascale Cossart. K+ efflux, is required for histone-H3 dephosphorylation by Listeria LLO and other pore forming toxins. Infect. Immun., Apr 2011; 10.1128/IAI.01243-10.
Norma Olivares-Zavaleta, Aaron Carmody, Ronald Messer, William M. Whitmire, and Harlan D. Caldwell. Chlamydia pneumoniae Inhibits Activated Human T Lymphocyte Proliferation by the Induction of Apoptotic and Pyroptotic Pathways. J. Immunol. 2011; 186:7120-7126.
Coming soon: Red FLICA™ Caspase-1 Reagents
Perform multiplexing studies with green-labeled biomarkers via flow cytometry, fluorescence plate reader, and fluorescence microscopy.
Join our mailing list to be informed when this new product becomes available!
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