How do I wash the cells?

The MitoPT™, FLICA™, FLISP™, and Cholinestease kits require a short wash step to remove any unbound background reagent. After incubating with the reagent, remove the media and spin with fresh media or ICT’s wash buffer. Adherent cells can be spun in a plate. If that is not feasible, or if your cells cannot withstand centrifugation, just replace the labeled media with fresh media or our wash buffer, and incubate 1 hour to allow any unbound reagent to diffuse back out of the cell. Remove this solution and analyze the cells. The MR kits do not require a wash step, so they may work best with sensitive cells, and frozen cells.