How much reagent do I use?

The reagents are provided as stabilized lyophilized powders. Just reconstitute the vial with ~50uL DMSO, dilute with ~300uL PBS, and then add ~10uL of the diluted reagent to your cells. Your particular cell line may require more or less reagent. ICT’s kits can be customized by adapting the amount of reagent used and the length of the incubation periods.