10x Phosphate Buffered Saline
Improve your ELISA using PB1
ICT’s 10X Phosphate Buffered Saline (PBS or PB1)
A well-tested formulation of buffers and salts designed to effectively balance the pH without disrupting ELISA binding reactions. Also use it as a base to create your own ELISA buffers or for other applications in the lab like dialyzing proteins and running samples over HPLC and Protein G columns. ICT's PB1 is supplied as a 10x concentrate to conserve valuable storage space. Simply add 9 parts deionized water to 1 part PBS, mix, and it’s ready to use. No pH adjustment is required. After dilution, 1x PBS will suppress bacterial growth for up to 6 months at room temperature and 12 months at 2°-8°C. The concentrated buffer will suppress bacterial growth for at least 12 months at room temperature and 18 months at 2°-8°C.
How do I use PBS?
Simply dilute PB1 1:10 (for example, add 100mL PB1 to 900mL diH20 for a final volume of 1000mL), let the solution stir for 15 minutes. As PB1 is concentrated, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil.
Plate Coating Buffers Block Buffers Conjugate Stabilizer Diluents
Sample Diluents Assay Diluents Wash Buffer
Substrates Stop Solutions Custom Lab Solutions
ICT has specifically formulated two plate coating buffers for use with antibodies and antigens. Both coating buffers stabilize coated proteins by maintaining their tertiary three-dimensional structure, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal.
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