Stop Solutions
Stop Solutions are used to prevent further color development of the substrate in an ELISA assay.
The chromogenic signal is arrested in endpoint assays so that the readout is indicative of the time the assay is stopped. Stop solution may also be added at the final time point in a kinetics assay.
ICT offers 3 reliable stop solutions:
Stop Solution for TMB Microwell Substrates (STOP1)
An easy-to-use liquid stop solution. STOP1 is suitable for use with our TMB substrates SUB1, SUB2, and SUB3. This TMB stop solution changes the chromogen from blue to yellow, and hence the absorbance from 620 nm to 450 nm.
Stop Solution for ABTS Microwell Substrates (STOP2)
An easy-to-use liquid stop solution. STOP2 is compatible with our ABTS substrate SUB4. This ABTS stop solution does not change the color or the absorbance of the chromogen, and so the absorbance is read at 405 nm to 410 nm.
Stop Solution for pNPP Microwell Substrates (STOP3)
An easy-to-use liquid stop solution. STOP3 is compatible with our alkaline phosphatase substrate SUB5. This pNPP stop solution does not change the color or the absorbance of the chromogen, and so the absorbance is read at 405 nm to 420 nm.
ICT has specifically formulated several buffers, diluents, and stabilizers for immunoassay development and manufacturing.
Why Develop it Yourself When We Have the Solution?
ELISA Solutions Application Guide
ELISA Solutions Product and Price List (PDF)
Order Form (Doc)
Additional Questions?
Call ICT Technical Support:
1-800-829-3194