Apoptosis Inducing Reagents

Apoptosis, a form of Programmed Cell Death (PCD), may be induced in experimental systems through a variety of methods. ICT offers a few select reagents to create apoptosis-positive controls for investigations of toxicology, therapeutic efficacy, and many other experiments where apoptosis is to be measured.

Staurosporine: isolated from Streptomyces, is a protein kinase inhibitor. Staurosporine induces DNA fragmentation and apoptosis in Jurkat cells at 1mM in 2-3 hours.

Camptothecin: isolated from the Chinese herb xi shu (Camptotheca acuminata), is an inhibitor of DNA topoisomerase I. Camptothecin induces apoptosis at 4mg/mL in Jurkat cells at S-phase of the cell cycle.

TNF-α: human recombinant protein expressed in E. coli as a single, non-glycosylated, polypeptide of 158 amino acids.

TNF-β: human recombinant protein expressed in E. coli as a single, non-glycosylated, polypeptide of 171 amino acids.

Measure the apoptotic response of experimental and control samples with ICT's range of fluorescent probes for intracellular protease detection and analysis:
in vitro caspase detection: FLICA | Magic Red
in vivo apoptosis detection: FLIVO
in vitro mitochondrial status: MitoPT