FLIVO® Kits

In vivo apoptosis detection tracers

Our proprietary in vivo apoptosis detection technology, FLIVO™, is a powerful tool for in vivo apoptosis detection in living animal models. Our fluorescent in vivo apoptosis detection tracers are non-toxic, cell-permeant reagents that specifically label apoptotic cells in vivo for analysis with fluorescence detection methods. The FLIVO tracer technology is based on a common caspase-inhibitor peptide sequence, VAD, and a fluoromethylketone moiety that form covalent bonds to intracellular active caspase enzymes involved in programmed cell death. The FLIVO apoptosis assays have been successfully used in a range of animal models to assess apoptosis as a result of a treatment or biological condition.

FLIVO™ tracers are available with visible fluorescence labels and near-infrared fluorescence labels. Our visible fluorescence FAM-FLIVO and SR-FLIVO in vivo apoptosis tracers utilize carboxyfluorescein (FAM) or sulforhodamine-B labels, respectively, for in vivo apoptosis labeling and ex vivo analysis of histological samples via optical imaging or flow cytometry. Our new NIR-FLIVO™ in vivo apoptosis tracers and free dye controls utilize the near-infrared fluorescent labels DyLight® 690 and DyLight® 747 for noninvasive in vivo imaging and apoptosis detection with small animal imaging systems.

Browse protocols and data on the product pages to learn more, and contact ICT to discuss how these in vivo apoptosis tracers would benefit your research.

FLIVO™ is a trademark of ImmunoChemistry Technologies, LLC.
DyLight® is a registered trademark or Thermo Fisher Scientific, Inc. and its subsidiaries.