Substrates for ELISA and Immunoblotting, TMB, ABTS, pNPP

Substrates for ELISA and Immunoblotting, TMB, ABTS, pNPP

Substrates are used to generate the read-out signal of an immunoassay utilizing an enzyme-labeled detection antibody. ICT offers several varieties of colorimetric alkaline phosphatase and HRP substrates for ELISA and immunoblotting applications, including TMB substrates for HRP detection with varying levels of sensitivity.

Format Conjugate    Substrate Absorbance
Microwell HRP TMB 1-Component HRP Microwell Substrate (SUBT), our most popular TMB substrate. This TMB substrate for HRP detection provides the reference for performance of all the other TMB substrates offered. TMB 1-Component HRP Microwell Substrate is ideal for most HRP detection ELISAs where the target is in the ng-pg range. We recommend starting with this TMB substrate formulation to estimate the level of sensitivity needed in the assay. 370 or 620-650 nm
Microwell HRP TMB Super Sensitive 1-Component HRP Microwell Substrate, our most sensitive TMB substrate, is approximately 40-fold more sensitive than our SUBT TMB substrate. This sensitive TMB substrate for ELISA is useful when detecting very low levels of a target molecule, with samples that must be highly diluted (1:10,000), to amplify the signal when using antibodies with low binding capacity, with samples that exhibit high steric hindrance, and to shorten the incubation time of the assay. 370 or 620-650 nm
Microwell HRP TMB Slow Kinetic 1-Component HRP Microwell Substrate exhibits roughly 25% less sensitivity than SUB1 TMB substrate. Lower sensitivity TMB substrates are ideal for HRP detection ELISAs where the test samples contain high levels of the target molecule, for assays with long incubation periods (such as overnight incubations), and for assays that simply do not require a high level of sensitivity. 370 or 620-650 nm
Microwell HRP ABTS 1-Component HRP Microwell Substrate is a good choice of HRP substrate for HRP detection-based assays with: samples containing high levels of the target molecule; samples that do not require much dilution (i.e. past 1:100); long incubation periods (such as overnight incubations); and for assays that simply do not require a high level of sensitivity. 405-410 nm
Microwell AP pNPP 1-Component AP Microwell Substrate for alkaline phosphatase detection (AP) in ELISA/EIA microwell formats. This formulation of pNPP alkaline phosphatase substrate contains a stabilizer to extend its benchtop shelf life. 405-420 nm
Immunoblotting
HRP TMB 1-Component HRP Membrane Substrate is useful for immunoblotting applications where HRP-conjugated molecules are used for detection. TMB membrane substrates react with HRP, yielding a dark blue reaction product. Our TMB 1-Component HRP Membrane Substrate formulation does not contain any aprotic solvents. 370 or 620-650 nm
Immunoblotting 
AP BCIP/NBT 1-Component AP Membrane Substrate contains BCIP (5-bromo-4-Chloro-3’-Indolyphosphate p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. BCIP/NBT 1-Component AP Membrane Substrate reacts with alkaline phosphatase, yielding a bluish-purple reaction product. NBT serves as the oxidant and BCIP is the alkaline phosphatase substrate.
370 nm

 

RELATED:

View our selection of Stop Solutions for ELISA and Membrane Substrates: STOP SOLUTIONS
Stabilize your HRP- or AP-Conjugated Secondaries with our CONJUGATE STABILIZER DILUENTS

7 Item(s)

per page

Set Descending Direction

7 Item(s)

per page

Set Descending Direction