SynBlock Blocking Buffer (BB3) is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.
Containing inert non-reactive blockers, SynBlock Blocking Buffer reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
SynBlock Blocking Buffer contains an antimicrobial agent for room temperature blocking and enables dried plates to be stored at 2°-8°C for up to 18 months. BB3 is formulated at 1X, ready to use, and is stable for 18 months when stored at 2°-8°C or 1 month when stored at room temperature.
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| Best For: | BB3 is used to block coated ELISA plates that require high to very high level blocking or that require a protein-free blocking buffer. |
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| pH: | 7.4 at 1x |
| Contains: | All synthetic materials
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| Supplied At: | 1x |
| Storage: | 2°-8° C, Ships Overnight (Domestic), 2-Day, 3-Day Shipping Available for orders placed by phone, fax, or email, International Priority Shipping |
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What does a block buffer do to the coated plate?
All of ICT's unique blocking buffers contain proprietary additives that stabilize the antigenic and functional regions of the coated protein by maintaining a level of hydration during the drying and storage process. These buffers also contain inert additives that block any "sticky" nonspecific binding regions of the adsorbed protein and any uncoated regions of the polystyrene plate surface. This reduces nonspecific binding of the conjugate and random deposits of sample proteins, reducing background noise and increasing the specific signal. ICT's proprietary block buffers also include an antimicrobial agent to prevent bacterial growth during the blocking process, which allows blocking to be done at room temperature, and to extend the shelf-life of the plates. Plates can be blocked and dried at room temperature and stored for 1 year or longer at 2°-8°C, depending on the coated protein.
When should BB3 be used?
BB3 is used as a high level blocker for sandwich ELISA formats that require high to very high level blocking, or that require a blocker that contains no proteins. ICT offers a trial pack containing 100 mL each of three different blocking buffers for an economical and fast method of selecting the best blocking buffer for a particular assay (BBOP, catalog #957).
What type of ELISA plates will BB3 work on?
BB3 works on all types of polystyrene plates except Immulon 2 plates. When blocking with BB3, ICT recommends Corning Costar® 96-Well EIA/RIA Stripwell™ Plates (ICT catalog #25).
How are plates dried?
Plates coated and blocked with ICT's reagents should always be dried at room temperature. Blocked plates should first be aspirated to remove Block Buffer. To dry, place them in a fume hood, loosely covered with foil. Turn the fume hood on and dry overnight. An alternate method is to dry the plates in a vacuum chamber without heat for 4 hours. Plates should never be stacked on one another while drying. It is recommended that lyophilization should not be used as a drying method.
How should dried plates be stored?
Dried plates should be placed in a foil pouch (ICT catalog # 6288) with a 5 gram desiccant packet (ICT catalog # 6289) and sealed. The plates can then be stored at 2°-8°C for up to one year.
