Blocking Buffers

Efficiently block assay wells and stabilize coated protein with ICT's Blocking Buffers

Once the antibody or protein antigens have been properly adsorbed onto the ELISA / EIA plate, the next critical step in creating a reliable assay is the blocking of the plate. Proper blocking of the unoccupied areas of the plate wells is paramount to attaining an accurate signal.

• Prevent nonspecific binding
• Reduce ELISA background signal
• Block nonspecific binding to adsorbed proteins
• Stabilize adsorbed protein for better interactions

ICT's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing the coated protein. Four formulations offer various benefits to different assay situations. Browse the product selections below, or order our Blocking Buffer Optimization Pack to try three formulations at an economical rate.

  General Blocker Blocking Buffer BB1

  Neptune Block Blocking Buffer BB2

  SynBlock Blocking Buffer BB3

  Phosph-Free Blocking Buffer BB4

  Blocking Buffer Optimization Pack (BBOP)