Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein.
HRP Conjugation Summary
Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4 fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Specificity Summary
Using ID, IEP, and ELISA analysis techniques, the IgG component of this conjugate was specific for normal human IgG and was non-reactive with k or l light chains. This conjugate is suitable for ELISA and Western blotting techniques.
Stability
This IgG-HRP anti-Human IgG conjugate should retain its effective activity for at least 1 year when stored at 2-8° C.
Precautions and Disclaimer
For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses.
| Product Datasheet: | HRP AffiPure Goat anti-Human IgG Fc Datasheet
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| Best For: | ELISA/ EIA and Western blotting |
| To Use: | Recommended Starting Dilutions: Western Blotting 1:500 – 1:2,000 Optimal conjugate dilutions should be determined for your particular assay conditions. For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses. |
- Bourdage, J.S., et al. Differential reactivity of anti-primate and anti-human secondary antibodies against human and monkey immunoglobulins: Implications for determining the sensitivity of immunogenicity assays. J. Immunol. Meth. 303(1-2), 76-80, (2005).
- Tomkowicz, B., et al. Interaction of endosialin/TEM1 with extracellular matrix proteins mediates cell adhesion and migration. PNAS 104(46), 17965-17970, (2007).
- Vangelista, L., et al. A minimal receptor-Ig chimera of human Fc€RI α-chain efficiently binds secretory and membrane IgE. Protein Eng. 15(1), 51-57, (2002).
| Storage: | 2°-8° C, Ships Overnight (Domestic), International Priority Shipping |
