PB1 10x Phosphate Buffered Saline

Catalog Number: 6252, 6157, 6158, 6159, 6160

Availability: In stock

$25.00

Quick Overview

PB1 is a well-tested liquid formulation of buffers and salts designed to effectively balance pH without disrupting antibody-sandwich ELISAs and antigen-down ELISA binding interactions. It may be used as a base to create custom-made ELISA buffers or for other applications in the lab, such as washing cells, protein dialysis, or running Protein A or Protein G columns. ICT's PB1 is supplied as a 10x liquid concentrate. Simply add 9 parts deionized water to 1 part 10X PBS, mix, and it is ready to use. No pH adjustment is required. PB1 does not contain any preservatives that may interfere with binding interactions.

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PB1 is a well-tested liquid formulation of buffers and salts designed to effectively balance pH without disrupting antibody-sandwich ELISAs and antigen-down ELISA binding interactions. It may be used as a base to create custom-made ELISA buffers or for other applications in the lab, such as washing cells, protein dialysis, or running Protein A or Protein G columns. ICT's PB1 is supplied as a 10x liquid concentrate. Simply add 9 parts deionized water to 1 part 10X PBS, mix, and it is ready to use. No pH adjustment is required. PB1 does not contain any preservatives that may interfere with binding interactions.
Product Datasheet:

PB1 Phosphate Buffered Saline, 10X Product Datasheet.pdf
Best For: useful as a buffer base for custom-made ELISA buffers; washing cultured cells; protein dialysis; running Protein A or Protein G columns
To Use: Simply dilute 10x PB1 1:10 (e.g. combine 100 mL of 10x PB1 and 900 mL diH2O, yielding a total volume of 1000 mL), let the solution stir for 15 minutes, and use. As PB1 is concentrated, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil.
pH: 7.4 at 1x
Contains: No proteins
Supplied At: 10x
Storage: CFSE at -20° C, International Priority Shipping
MSDS:

PB1MSDS.pdf
Certificates of Analysis: PB1_9N28.jpg

What is the shelf-life of PB1?
When refrigerated, 10X PB1 has a shelf-life of 2 years. When stored at room temperature, we recommend using the concentrated 10X PB1 within 6 months. After dilution, 1X PB1 may be stored at 2°-8°C for 2 weeks, or 5 days at room temperature. PB1 does not contain any preservatives.

How can I modify the 1X PBS to make a long-term buffer to store my different gel matrices, and column materials?
Simply add sodium azide powder (NaN3) at 0.1% w/v NaN3 concentration in 1X PBS. For example, add 1g NaN3 and 100mL 10X PBS to 900mL diH20 for a final volume of 1L. This buffer may retard bacterial growth long-term (6 months at room temperature, or 2 years refrigerated). Care should be taken when handling NaN3 powder due to its cross-functional toxicity potential.