Rapid Test Mouse Monoclonal Isotyping Kit

Catalog Number: 998

Availability: In stock

$199.00

Quick Overview

The ICT Rapid Test Mouse Monoclonal Isotyping Kit is a five-minute rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. This sensitive, gold-based rapid assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid.



  • Reduce prep & runtime – test produces result in under 20 minutes

  • Sensitive – gold-sol-based assay demonstrates increased sensitivity over latex-bead assay

  • Convenient – test for 6 major isotypes with one 5-minute rapid test

  • Ready-to-use – kit provides fully-prepared reagents and test strips


Each Isotyping Kit contains materials for testing ten samples. A total of ten (10) pouches, each containing a cassette for detecting IgG1, IgG2a, and IgG2b isotypes and a cassette for detecting IgG3, and IgM, and IgA antibodies are included.


Order Rapid Test Mouse Monoclonal Isotyping Kit (cat. #998) from this site, or call 1-800-829-3194 to place your order!

ICT's Rapid Test Mouse mAb Isotyping Kit contains ready-to-use cassettes to rapidly determine mAb class and subclass.

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  • ICT's Rapid Test Mouse mAb Isotyping Kit contains ready-to-use cassettes to rapidly determine mAb class and subclass.
The ICT Rapid Test Mouse mAb Isotyping Kit is a five-minute rapid lateral flow assay with ELISA sensitivity for monoclonal antibody class and subclass determination. This sensitive, gold-based rapid assay can be run on both tissue culture supernatant fluid and on mouse ascites fluid.

Determining the class and subclass of a monoclonal antibody is useful in determining the best immunoglobulin purification method. For example, IgA and IgM are often best purified by size (gel exclusion) or on immuno affinity separation columns, whereas IgG2a and IgG2b can be purified on protein A at a pH of 7 to 8. IgG1 binds best to protein A at a pH of 8 to 9. In addition, each class and isotype can be digested to Fab fragments using the appropriate amount of pepsin or other enzymes for each isotype or antibody class. Our rapid Mouse Monoclonal Antibody Isotyping Kit reduces a day-and-a-half procedure to a five-minute assay.

The Rapid Test Mouse Monoclonal Isotyping Kit is also useful for assessing the purity of a self-purified or purchased monoclonal antibody; multiple lines appear on the test strips to indicate subclass contamination.

Each Isotyping Kit contains ten (10) pouches for testing ten samples. A total of ten (10) cassettes for detecting IgG1, IgG2a, and IgG2b isotypes and ten (10) cassettes for detecting IgG3, and IgM, and IgA antibodies are included. The kit also contains one (1) 45 ml bottle of Sample Diluent.

Product Datasheet:

ISO1_insert.pdf
Best For: Determining the class and subclass of a mononclonal antibody, assessing the purity of the monoclonal antibody, and reducing assay runtime.
To Use: When a properly diluted sample containing a specific isotype is added to the sample-well, specific-class and subclass soluble complexes are formed with the embedded gold conjugates. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody impregnated membrane. Typically, when antibodies are tested at 10 nanograms per milliliter, results are read at five (5) to ten (10) minutes. Results should not be read after 10 minutes.

Procedure for monoclonal antibody ascites fluid:
For ascites fluid, the darker red line indicates the class or subclass antibody present. Weak red lines often appear on other isotypes indicating the presence of host serum immunoglobulins in the ascites.

  1. Add 0.5 µl of ascites fluid to 4 ml of Sample Diluent - vortex to mix
  2. Add 150 µl of diluted ascites fluid to the Sample Well (S).
  3. Wait 5 (five) minutes
  4. Read results – the darker line is the isotype
NOTE: Do not read after ten (10) minutes.

Procedure for cell culture fluid:
For supernatants, a dark red line indicates which isotype or class-specific antibody is present. In very few instances, weak red lines may appear on one or more additional isotype(s) indicating multiple hybridoma clones.

  1. Add 5.0 µl of supernatant fluid to 0.5 ml of Sample Diluent - vortex to mix
  2. Add 150 µl of diluted supernatant fluid to Sample Well (S)
  3. Wait 5 (five) minutes
  4. Read results
NOTE: Do not read after ten (10) minutes.

Note: For supernatants that contain the monoclonal antibody at less than 1 microgram per ml, dilute the sample 1:10 by adding 55 µl of supernatant to 500 µl of sample diluent and vortex to mix. Perform Steps 2 through 4.
Contains: Ten (10) pouches and Sample Diluent (45 ml)
Each pouch contains the following:
  • 1 cassette with strips impregnated with anti-IgG1, anti-IgG2a, and anti-IgG2b isotypes
  • 1 cassette with strips impregnated with anti-IgG3, anti-IgM, and anti-IgA isotypes
Storage: Room Temperature, Ships Overnight (Domestic), 2-Day, 3-Day Shipping Available for orders placed by phone, fax, or email, International Priority Shipping
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