Goats were immunized with a purified preparation of mouse IgG Fc fragment that was obtained from a purified normal mouse IgG antibody protein pool. The hyperimmune IgG fraction was initially pre-adsorbed over solid phase gels bearing covalently coupled mouse Fab protein. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled mouse IgG Fc protein.
HRP Conjugation Summary
Affinity purified, anti-mouse IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-mouse IgG Fc prep just prior to its reaction with a 4 fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Specificity Summary
Using ID and IEP analysis techniques, the IgG component of this conjugate was specific for normal mouse IgG and was non-reactive with IgM or IgE serum proteins. This conjugate is suitable for ELISA, Western blotting, and histology techniques.
Stability
This IgG-HRP anti-Mouse IgG conjugate should retain its effective activity for at least 1 year when stored at 2-8° C.
Precautions and Disclaimer
For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses.
| Product Datasheet: | HRP AffiPure Goat anti-Mouse IgG Fc Datasheet
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| Best For: | ELISA/ EIA and Western blotting |
| To Use: | Recommended Starting Dilutions: Western Blotting 1:500 – 1:2,000 Optimal conjugate dilutions should be determined for your particular assay conditions. For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses. |
- Honda, S.I. et al. Enhanced humoral immune responses against T-independent antigens in Fcα/µR-deficient mice. PNAS, 106 (27), 11230-11235, (2009).
- Plumer, A. et al. Development of fragment-specific osteopontin antibodies and ELISA for quantification in human metastatic breast cancer. BMC Cancer, 8, 38-49, (2008).
- Gils, A. et al. Development of a genotype 325-specific proCPU/TAFI ELISA. Arterioscler. Thromb. Vasc. Biol. 23, 1122-1127, (2003).
| Storage: | 2°-8° C, Ships Overnight (Domestic), International Priority Shipping |
