Goats were immunized with a purified preparation of rabbit IgG Fc fragment that was obtained from a purified normal rabbit IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled rabbit IgG Fc protein.
HRP Conjugation Summary
Affinity purified, anti-rabbit IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-rabbit IgG Fc prep just prior to its reaction with a 4 fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Specificity Summary
Using ELISA and IEP analysis techniques, the IgG component of this conjugate was specific for normal rabbit serum IgG and was non-reactive with other serum proteins. This conjugate is suitable for ELISA and Western blotting analysis techniques.
Stability
This IgG-HRP anti-Rabbit IgG conjugate should retain its effective activity for at least 1 year when stored at 2-8° C.
Precautions and Disclaimer
For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses.
| Product Datasheet: | HRP AffiPure Goat anti-Rabbit IgG Fc Datasheet
|
| Best For: | ELISA/ EIA and Western blotting |
| To Use: | Recommended Starting Dilutions: Western Blotting 1:500 – 1:2,000 Optimal conjugate dilutions should be determined for your particular assay conditions. For research and manufacturing use only. Not for use in diagnostic applications, nor for drug, household or other uses. |
- Carthew, P., et al. Comparison of alkaline phosphatase and horseradish peroxidase conjugated antisera in the ELISA test for antibodies to reovirus 3, mouse hepatitis, and Sendai viruses. Lab. Animal, 15, 69-73, (1981).
- Shafat, I., et al. An ELISA method for the detection and quantification of human heparanase. Biochem. Biophys. Res. Commun. 341(4), 958-963, (2006).
- Tong, Z.B., et al. Developmental expression and subcellular localization of mouse MATER, an oocyte-specific protein essential for early development. Endocrinology, 145(3), 1427-1434, (2004).
- Moir, R.D., et al., Autoantibodies to redox-modified oligomeric Aβ are attenuated in the plasma of Alzheimer’s disease patients. J. Biol. Chem. 280(17), 17458-17463, (2005).
| Contains: | 1.0 mg IgG conjugated to 2-4 HRP enzymes per IgG molecule. Conjugate is dissolved in a Tris-based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent. |
| Storage: | 2°-8° C, Ships Overnight (Domestic), International Priority Shipping |
