In June, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of June citations from 2021.
Santucci, P;Greenwood, DJ;Fearns, A;Chen, K;Jiang, H;Gutierrez, MG. Intracellular localisation of Mycobacterium tuberculosis affects efficacy of the antibiotic pyrazinamide.
Nature communications. 2021 June 21; doi: 10.1038/s41467-021-24127-3. Article
"Stained cells were then washed twice and re-incubated in complete RPMI medium containing 1 µg/ml Hoechst 33342 (Immunochemistry Technologies, 639)
and live-cell imaging was further performed using the OPERA Phenix microscope with a ×40 water-immersion objective."
Xu, C;Sun, S;Johnson, T;Qi, R;Zhang, S;Zhang, J;Yang, K. The glutathione peroxidase Gpx4 prevents lipid peroxidation and ferroptosis to sustain Treg cell activation and suppression of antitumor immunity.
Cell reports. 2021 June 15; doi: 10.1016/j.celrep.2021.109235. Article
"For staining mitochondria, lymphocytes were incubated for 30 min at 37°C with 10 nM MitoTracker Deep Red (Life Technologies) or 20 nM TMRM (tetramethyl rhodamine, methyl ester; ImmunoChemistry Technologies)
after staining surface markers"
Park, SS;Gonzalez-Juarbe, N;Riegler, AN;Im, H;Hale, Y;Platt, MP;Croney, C;Briles, DE;Orihuela, CJ. Streptococcus pneumoniae binds to host GAPDH on dying lung epithelial cells worsening secondary infection following influenza.
Cell reports. 2021 June 15; doi: 10.1016/j.celrep.2021.109267. Article
"The Cell pellets were washed three times with PBS and incubated at room temperature for 30 min with 50 μg / ml of FITC-PspA and necrotic or late apoptosis cell death was stained by propidium iodide (PI) (ImmunoChemistry Technologies, USA)
according to ..."
Wan, F;Zhong, G;Wu, S;Jiang, X;Liao, J;Zhang, X;Zhang, H;Mehmood, K;Tang, Z;Hu, L. Arsenic and antimony co-induced nephrotoxicity via autophagy and pyroptosis through ROS-mediated pathway in vivo and in vitro.
Ecotoxicology and environmental safety. 2021 June 21; doi: 10.1016/j.ecoenv.2021.112442. Full Text
"Flow cytometry (FCM) to detect pyroptotic cells. To assess pyroptosis in TCMK-1 cells, Caspase-1 activity was determined using the Pyroptosis/Caspase-1 Assay (ImmunoChemistry Technologies, USA)
Chen, S;Luo, Z;Chen, X. Hsa_circ_0044235 regulates the pyroptosis of rheumatoid arthritis via MiR-135b-5p-SIRT1 axis.
Cell cycle (Georgetown, Tex.). 2021 June 7; doi: 10.1080/15384101.2021.1916272. Abstract
"Caspase-1 assay could indicate the degree of cell pyroptosis. Caspase-1 contents were assessed by flow cytometry and immunofluorescence staining. FLICA 660 Caspase-1 Assay Kit (9122, ImmunoChemistry Technologies, USA)
was used to perform flow cytometry."
Tamai, R;Mashima, I;Kiyoura, Y. Alendronate Augments Lipid A-Induced IL-1α Release via Activation of ASC but Not Caspase-11.
Inflammation. 2021 June 2; doi: 10.1007/s10753-021-01489-w. Abstract
"we performed a fluorochrome inhibitor of caspase assay (FLICA) using the caspase-8 staining reagent carboxyfluorescein-LETD-fluoromethylketone (FAM-LETD-FMK)
, according to the manufacturer's instructions (Immunochemistry Technologies, LLC; Bloomington, MN)."
Semlali, A;Beji, S;Ajala, I;Rouabhia, M. Effects of tetrahydrocannabinols on human oral cancer cell proliferation, apoptosis, autophagy, oxidative stress, and DNA damage. Archives of oral biology. 2021 June 16; doi: 10.1016/j.archoralbio.2021.105200. Text
"Following this culture period, the cells were exposed or not to 10 μM of either Δ 9 -THC or Δ 8 -THC for 24 h. In the first set of experiments, cellular ROS
production was determined using a specific kit assay from ImmunoChemistry Technologies (Bloomington, MN)"
MitoPT TMRM Assay
Propidium Iodide Stain
Flica 660 Caspase-1 Assay
FAM FLICA Caspase-8 Assay
Intracellular Total ROS Activity Assay