In November, researchers around the world cited ImmunoChemistry Technologies’ products in their publications. Explore our selection of November citations from 2017. Hoyle NP, Seinkmane E, Putker M, Feeney KA, Krogager TP, Chesham JE, Bray LK, Thomas JM, Dunn K, Blaikley J, O'Neil, JS. Circadian actin dynamics drive rhythmic fibroblast mobilization during wound healing. Sci Transl Med. 2017.Nov 8;9(415). pii: eaal2774. doi: 10.1126/scitranslmed.aal2774. Abstract "Collagen deposition was measured after 14 days by staining the sections for 3 hours with Col-F (ImmunoChemistry Technologies)." Tsai F, Homan PJ, Agrawal H, Misharin AV, Abdala-Valencia H, Haines GK, Dominguez S, Bloomfield CL, Saber R, Chang A, Mohan C, Hutcheson J, Davidson A, Budinger GRS,Bouillet P,Dorfleutner A,Stehlik C,Winter DR, Cuda CM, Perlman,H. Bim suppresses the development of SLE by limiting myeloid inflammatory responses. J Exp Med. 2017. Dec 4;214(12):3753-3773. doi: 10.1084/jem.20170479. Epub 2017 Nov 7. Abstract "Immulon 2 HB 96-well flat bottom plates (ImmunoChemistry Technologies) were precoated with methylated BSA followed by immobilization of antigens including dsDNA, ssDNA, histones, or dsDNA and histones (nucleosomes)." Mishra SK, Gao YG, Deng Y, Chalfant CE, Hinchcliffe EH, Brown RE. CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation. Autophagy. 2017. Nov 22:1-46. doi: 10.1080/15548627.2017.1393129. . Abstract "...Activated CASP1 levels were also assessed by western immuno-blotting of cell supernatant against CASP1-p20-specific antibody as well as with FLICA 660-YVAD-FMK detection reagent (ImmunoChemistry Technology, 9122) which consists of active …" Lin JX, Du N, Li P, Kazemian M, Gebregiorgis T, Spolski R, Leonard WJ. Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells. Nat Commun. 2017. Nov 6;8(1):1320. doi: 10.1038/s41467-017-01477-5. Full Text "To measure caspase activity in the cells, total splenocytes were incubated at 37 °C for 1 h with fluorescent-labelled caspase inhibitor FAM-VAD-FMK probes (FLICA reagent) for Poly caspases (91), caspase-1 (97), caspases-3, 7 (93), and caspase-9 (912) according to the manufacturer’s instructions (Immunochemistry Technologies, Bloomington, MN, USA). Data were acquired using a FACSCanto II flow cytometer (BD Immunocytometry Systems) and analysed using FlowJo (v9.7.5, Tree Star, Inc., Ashland, OR)." Chen J, Ou Y, Li Y, Hu S, Shao LW, Liu Y. Metformin extends C. elegans lifespan through lysosomal pathway. Elife. 2017.Oct 13;6. pii: e31268. doi: 10.7554/eLife.31268. Full Text "Magic Red stain (ImmunoChemistry Technologies #938, Bloomington, Minnesota) can be cleaved by cathepsin B, and generate red fluorescent substrate in functional lysosomes." Products Used:

Col-F Collagen Binding Reagent

Immulon™ 2 HB 96-Well Microtiter EIA Plate

FLICA 660 Caspase-1 Assay FAM-FLICA Poly Caspase Assay FAM-FLICA Caspase-1 Assay FAM-FLICA Caspase-3/7 Assay FAM-FLICA Caspase-9 Assay Magic Red Cathepsin B Assay