Pyroptosis is a highly inflammatory form of programmed cell death frequently triggered upon infection with intracellular pathogens.
This process begins with recognition of intracellular and extracellular danger signals, which leads to formation of a large, multi-protein complex known as the inflammasome. In this webinar, learn about the mechanisms of pyroptosis, including the critical role of caspase-1, a protease involved in activation of pro-inflammatory cytokines, plasma membrane rupture, and other morphological characteristics of pyroptosis. We’ll examine how ImmunoChemistry Technologies’ FLICA (Fluorescent-Labeled Inhibitor of Caspases) assays can be used to assess cell death, including caspase-1 activation and pyroptosis. We’ll also introduce our new FAM-FLICA WEHD Assay, which allows researchers another target sequence option for detecting caspase-1 in vitro.