Antibody Specific Active Caspase 3 Fluorescein Isothiocyanate (FITC) Detection Kit is highly specific for active human and murine. The kit utilizes a rabbit affinity purified polyclonal antibody against a 163-175.
Cell Technology's APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3. This neo epitope is present on the p18 subunit of cleaved caspase 3. Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
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