FAM-FLICA® Caspase-3/7 Assay Kit

from ImmunoChemistry Technologies Product Manual Safety Data Sheet
8 Citations
Detect caspase-3/7 activity with the FLICA Caspase-3/7 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

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FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit

FAM-FLICA® Caspase-3/7 Assay Kit
SKU: 93

Size: 25 Tests
Price:
$201
Caspases play important roles in apoptosis and inflammation. ICT’s FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-3/7 assay probe allows researchers to assess caspase 3 and 7 activation. The FLICA reagent FAM-DEVD-FMK enters each cell and irreversibly binds to active caspases 3 and 7. Because the FAM-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound FAM-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively.
FAM-DEVD-FMK
Caspase-3/7
488 nm / 530 nm
Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples and controls
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
  3. Reconstitute FLICA with 50 µL DMSO.
  4. Dilute FLICA 1:5 by adding 200 µL PBS.
  5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells).
  6. Incubate approximately 1 hour.
  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
  8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
  9. If desired, fix cells.
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm.

If working with adherent cells, please see the manual for additional protocols.

Kit 93: 25 Tests
  • FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial, #653
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
    • Kit 94: 100 Tests
  • FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials, #653
  • 10X Apoptosis Wash Buffer, 60 mL, #634
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual

    • Sun J, Huang Y, Gong J, Wang J, Fan Y, Cai J, Wang Y, Qiu Y, Wei Y, Xiong C, Chen J, Wang B, Ma Y, Huang L, Chen X, Zheng S, Huang W, Ke Q, Wang T, Li X, Zhang W, Xiang AP, Li W. Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice. Nat Commun. 2020 Oct 15;11(1):5196. doi: 10.1038/s41467-020-19042-y. Full Text

    "Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens;Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens..."

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