- Prepare samples and controls
- Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
- Reconstitute FLICA with 50 µL DMSO.
- Dilute FLICA 1:5 by adding 200 µL PBS.
- Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells).
- Incubate approximately 1 hour.
- Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
- If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
- If desired, fix cells.
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm.
If working with adherent cells, please see the manual for additional protocols.
Sun J, Huang Y, Gong J, Wang J, Fan Y, Cai J, Wang Y, Qiu Y, Wei Y, Xiong C, Chen J, Wang B, Ma Y, Huang L, Chen X, Zheng S, Huang W, Ke Q, Wang T, Li X, Zhang W, Xiang AP, Li W. Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice. Nat Commun. 2020 Oct 15;11(1):5196. doi: 10.1038/s41467-020-19042-y. Full Text
"Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens;Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the in situ cell death detection kit (Roche) or fluorescently labeled inhibitor of caspases (FLICA) probe active caspase-3 (ImmunoChemistry Technologies)/NeuN coimmunostaining was applied to monitor the general level of apoptosis in the ischemic hemisphere according to the manufacturer’s instructions. Fluorescent intens..."
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