FAM-FLICA® Caspase-3/7 Assay Kit

from ImmunoChemistry Technologies Product Manual Safety Data Sheet
16 Citations
Detect caspase-3/7 activity with the FLICA Caspase-3/7 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

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FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit
FAM-FLICA® Caspase-3/7 Assay Kit

FAM-FLICA® Caspase-3/7 Assay Kit
SKU: 93

Size: 25 Tests
Price:
$209

Bulk Order FAM-FLICA® Caspase-3/7 Assay Kit

Caspases play important roles in apoptosis and inflammation. ICT’s FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-3/7 assay probe allows researchers to assess caspase 3 and 7 activation. The FLICA reagent FAM-DEVD-FMK enters each cell and irreversibly binds to active caspases 3 and 7. Because the FAM-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound FAM-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively.
FAM-DEVD-FMK
Caspase-3/7
488 nm / 530 nm
Flow Cytometer, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples and controls
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
  3. Reconstitute FLICA with 50 µL DMSO.
  4. Dilute FLICA 1:5 by adding 200 µL PBS.
  5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells).
  6. Incubate approximately 1 hour.
  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
  8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
  9. If desired, fix cells.
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm.

If working with adherent cells, please see the manual for additional protocols.

Kit 93: 25 Tests
  • FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 1 vial, #653
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
    • Kit 94: 100 Tests
  • FLICA Caspase-3/7 Reagent (FAM-DEVD-FMK), 4 vials, #653
  • 10X Apoptosis Wash Buffer, 60 mL, #634
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual

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    He, L;Wei, T;Huang, Y;Zhang, X;Zhu, D;Liu, H;Wang, Z. miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury. Journal of Immunology Research. 2022 August 22; doi: 10.1155/2022/1642896. Full Article

    Su, M;Chen, C;Li, S;Li, M;Zeng, Z;Zhang, Y;Xia, L;Li, X;Zheng, D;Lin, Q;Fan, X;Wen, Y;Liu, Y;Chen, F;Luo, W;Bu, Y;Qin, J;Guo, M;Qiu, M;Sun, L;Liu, R;Wang, P;Hwa, J;Tang, WH. Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis. Nature cardiovascular research. 2022 August 4; doi: 10.1038/s44161-022-00108-7. Citation

    Kawashima, A;Kiriya, M;En, J;Tanigawa, K;Nakamura, Y;Fujiwara, Y;Luo, Y;Maruyama, K;Watanabe, S;Goto, M;Suzuki, K. Genome-wide screening identified SEC61A1 as an essential factor for mycolactone-dependent apoptosis in human premonocytic THP-1 cells. PLoS neglected tropical diseases. 2022 August 1; doi: 10.1371/journal.pntd.0010672. Article

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    Li, J;Diamante, G;Ahn, I;Wijaya, D;Wang, X;Chang, C;Ha, S;Immadisetty, K;Meng, H;Nel, A;Yang, X;Tian, X. Determination of the Nanoparticle- and Cell-Specific Toxicological Mechanisms in 3d Liver Spheroids Using Scrnaseq Analysis. SSRN Electronic Journal. 2022 June 18; doi: 10.2139/ssrn.4139095. Full Abstract

    Sun J, Huang Y, Gong J, Wang J, Fan Y, Cai J, Wang Y, Qiu Y, Wei Y, Xiong C, Chen J, Wang B, Ma Y, Huang L, Chen X, Zheng S, Huang W, Ke Q, Wang T, Li X, Zhang W, Xiang AP, Li W. Transplantation of hPSC-derived pericyte-like cells promotes functional recovery in ischemic stroke mice. Nat Commun. 2020 Oct 15;11(1):5196. doi: 10.1038/s41467-020-19042-y. Full Text

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    Happonen E, Tamarov K, Martikainen MV, Ketola K, Roponen M, Lehto VP, Xu W. Thermal dose as a universal tool to evaluate nanoparticle-induced photothermal therapy. Int J Pharm. 2020 Jul 16;587:119657. doi: 10.1016/j.ijpharm.2020.119657. Online ahead of print. Abstract

    Wang X, Chang CH, Jiang J, Liu X, Li J, Liu Q, Liao YP, Li L, Nel AE, Xia T. Mechanistic Differences in Cell Death Responses to Metal-Based Engineered Nanomaterials in Kupffer Cells and Hepatocytes. Small. 2020 May;16(21):e2000528. doi: 10.1002/smll.202000528. Epub 2020 Apr 26. Abstract

    Bank U, Deiser K, Plaza-Sirvent C, Osbelt L, Witte A, Knop L, Labrenz R, Jänsch R, Richter F, Biswas A, Zenclussen AC, Vivier E, Romagnani C, Kühl AA, Dunay IR, Strowig T, Schmitz I, Schüler T. c-FLIP is crucial for IL-7/IL-15-dependent NKp46+ ILC development and protection from intestinal inflammation in mice. Nat Commun. 2020 Feb 26;11(1):1056. doi: 10.1038/s41467-020-14782-3. Full Text

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    Lin JX, Du N, Li P, Kazemian M, Gebregiorgis T, Spolski R, Leonard WJ. Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells. Nat Commun. 2017. Nov 6;8(1):1320. doi: 10.1038/s41467-017-01477-5. Full Text

    Whang J, Back YW, Lee KI, Fujiwara N, Paik S, Choi CH, Park JK, Kim HJ. Mycobacterium abscessus glycopeptidolipids inhibit macrophage apoptosis and bacterial spreading by targeting mitochondrial cyclophilin D. Cell Death Dis. 2017 Aug 24;8(8):e3012. doi: 10.1038/cddis.2017.420. Article

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