Detect caspase-9 activity with the FLICA Caspase-9 Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.

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FAM-FLICA® Caspase-9 Assay Kit
SKU: 912

Size: 25 Tests
Price:
Sale price$201


Bulk Order FAM-FLICA® Caspase-9 Assay Kit

Caspases play important roles in apoptosis and inflammation. ICT’s FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-9 assay probe allows researchers to assess caspase-9 activation. The FLICA reagent FAM-LEHD-FMK enters each cell and irreversibly binds to activated caspase-9. Because the FAM-LEHD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound FAM-LEHD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase-9 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively.
FAM-LEHD-FMK
Caspase-9
488 nm / 530 nm
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples and controls
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
  3. Reconstitute FLICA with 50 μL DMSO.
  4. Dilute FLICA 1:5 by adding 200 μL PBS.
  5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 μL to 290 μL of cultured cells).
  6. Incubate approximately 1 hour.
  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
  8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
  9. If desired, fix cells.
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM FLICA excites at 492 nm and emits at 520 nm.

If working with adherent cells, please see the manual for additional protocols.

Kit 912: 25 Tests
  • FLICA Caspase-9 Reagent (FAM-LEHD-FMK), 1 vial, #677
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Kit 913: 100 tests
  • FLICA Caspase-9 Reagent (FAM-LEHD-FMK), 4 vials, #677
  • 10X Apoptosis Wash Buffer, 60 mL, #634
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Krętowski, R;Cechowska-Pasko, M. The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells. International journal of molecular sciences. 2022 August 18; doi: 10.3390/ijms23169285. Full Abstract

    Trugilho, MRO;Azevedo-Quintanilha, IG;Gesto, JSM;Moraes, ECS;Mandacaru, SC;Campos, MM;Oliveira, DM;Dias, SSG;Bastos, VA;Santos, MDM;Carvalho, PC;Valente, RH;Hottz, ED;Bozza, FA;Souza, TML;Perales, J;Bozza, PT. Platelet proteome reveals features of cell death, antiviral response and viral replication in covid-19. Cell death discovery. 2022 July 16; doi: 10.1038/s41420-022-01122-1. Full Text

    Ophélie, G;Fabien, B;Guillemine, D;Patrice, G;Stéphane, M;Megi, B;Marie-Hélène, E;Sabrina, L;Etienne, G;Christelle, G;Isabelle, B;Baudrimont, M. Cellular and molecular mechanisms of NiONPs toxicity on eel hepatocytes HEPA-E1: An illustration of the impact of Ni release from mining activity in New Caledonia. Chemosphere. 2022 May 28; doi: 10.1016/j.chemosphere.2022.135158. Article

    Kubiak, AB;Ziółkowska, EI;Korycka-Wołowiec, AB;Robak, T;Wołowiec, D. The influence of venetoclax, used alone or in combination with cladribine (2-CdA), on CLL cells apoptosis in vitro: Preliminary results. Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University. 2022 April 25; doi: 10.17219/acem/148142. Abstract

    Xu P, Wang X, Li Y, Qiu J. Establishment of a Parvovirus B19 NS1-Expressing Recombinant Adenoviral Vector for Killing Megakaryocytic Leukemia Cells. Viruses. 2019 Sep 4;11(9). pii: E820. doi: 10.3390/v11090820. Abstract

    Zhai Z, Liu W, Kaur M, Luo Y, Domenico J, Samson JM, Shellman YG, Norris DA, Dinarello CA, Spritz RA, Fujita M. NLRP1 promotes tumor growth by enhancing inflammasome activation and suppressing apoptosis in metastatic melanoma. Oncogene. 2017. Abstract.

    Whang J, Back YW, Lee KI, Fujiwara N, Paik S, Choi CH, Park JK, Kim HJ. Mycobacterium abscessus glycopeptidolipids inhibit macrophage apoptosis and bacterial spreading by targeting mitochondrial cyclophilin D. Cell Death Dis. 2017 Aug 24;8(8):e3012. doi: 10.1038/cddis.2017.420. Article

    Lin JX, Du N, Li P, Kazemian M, Gebregiorgis T, Spolski R, Leonard WJ. Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells. Nat Commun. 2017. Nov 6;8(1):1320. doi: 10.1038/s41467-017-01477-5. Full Text

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