Apoptosis and Autophagy Kits

66 products

Apoptosis is a process of programmed cell death that occurs in multicellular organisms. It is a highly regulated and controlled process that occurs normally during development and aging as a homeostatic mechanism to maintain cell populations.

Autophagy plays a critical role in maintaining homeostasis by preventing the accumulation of damaged organelles by disassembling unnecessary or dysfunctional cells and cellular components. Autophagy occurs at low levels in the cell under normal conditions and can be rapidly upregulated during times of starvation or stress.

ICT offers a full line of products to induce apoptosis, including FLICA, FLIVO, Annexin kits and more; our Autophagy Assay - Red Fluorescent enables researchers to detect and monitor the in vitro development of autophagy in living cells.

Showing 25 - 48 of 66 products

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, then stained with Chicken Annexin V-Fluorescein Assay (Cat. 9140). Chicken Annexin V-Fluorescein labeled only 16.9% of control cells (UR + LR = 13.2% + 3.7%, left); staurosporine induced Chicken Annexin V-Fluorescein labeling in 97% of cells (UR + LR = 46.6% + 50.4%, right). Staurosporine increased the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Murphy, ICT, 227:19.

ImmunoChemistry Technologies Chicken Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), then stained with Canine Annexin V-Fluorescein Assay (cat. 9139). Annexin V-Fluorescein labeled only 15.6% of the control cells (UR + LR = 9.8% + 5.8%, left); staurosporine induced Annexin V-Fluorescein labeling in 97.3% of cells (UR + LR = 11.5% + 85.8%, right). Staurosporine did not significantly increase the proportion of dually stained cells (UR). Data courtesy of Mrs. T. Murphy, ICT, 224:69-71.

ImmunoChemistry Technologies Canine Annexin V-Fluorescein Apoptosis Assay Kit

$455

Figure 1. Jurkat cells were treated with a negative control (left) or staurosporine (right), washed, and then stained with Bovine Annexin V-Fluorescein (Cat. 9138). Bovine Annexin V-Fluorescein labeled only 17.6% of control cells (UR + LR = 13.5% + 4.1%, left); staurosporine induced Bovine Annexin V-Fluorescein labeling in 98.8% of cells (UR + LR = 15.3% + 83.5%, right). Staurosporine did not increase the proportion of dual stained cells (UR). Data courtesy of Mrs. Tracy Hanson, ICT, 224:69-71.

ImmunoChemistry Technologies Bovine Annexin V-Fluorescein Apoptosis Assay Kit

$455

ImmunoChemistry Technologies Staurosporine

$91.50

ImmunoChemistry Technologies Camptothecin

From $33.25

Figure 1. Jurkat cells were treated with staurosporine (Positive, Induced, right histogram). Negative control cells were spiked with a DMSO vehicle control (Negative, Non-induced, left histogram). Cells were stained with SR-VAD-OPH for 1 hour, washed twice, and read on a flow cytometer. Only 8.0% of control cells displayed caspase activity, whereas staurosporine induced caspase activity in 90.3% of the experimental cells. This is a ratio of 11:1. Data courtesy of Ms. Tracy Hanson, ICT 092713.

Figure 2. Jurkat cells were treated with staurosporine to induce apoptosis, then labeled with SR-VAD-OPH for 60 minutes. Cells were washed twice and wet-mount slides were prepared. A grey DIC image (left) was taken, and red fluorescence (right) was detected using a band pass filter (excitation at 565 nm, emission at 590-600 nm). SR-VAD-OPH reveals complete caspase activation in the form of red fluorescence in all cells. Data courtesy of Ms. Tracy Hanson, ICT (ICT111809; SR_OPH_111_17_18).

ImmunoChemistry Technologies Red Fluorescent SR-VAD-OPH in vitro Poly Caspase Apoptosis Detection Reagent

$555

Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, and then labeled with FAM-DEVD-OPH (cat. 6356). Wet-mount slides were prepared and green fluorescence (right) was detected using a band pass filter (excitation at 488 nm, emission at 520 nm). FAM-DEVD-OPH reveals complete caspase-3/7 activation in the form of green fluorescence in all cells. The corresponding DIC image is shown (left). Data courtesy of Ms. Tracy Hanson, ICT (ICT111709; FAM-DEVD-OPH_in_59_60).

ImmunoChemistry Technologies Green Fluorescent FAM-DEVD-OPH in vitro caspase-3/7 Apoptosis Detection Reagent

$555

Figure 1. Jurkat cells were treated with DMSO (left) or staurosporine (right), labeled with FAM-VAD-OPH II, washed, and stained with PI. Four cell populations are seen: unstained live cells do not fluoresce (lower left); early apoptotic cells fluoresce green with FAM- VAD-OPH II (lower right); late apoptotic cells (upper right) fluoresce green (active caspases) and red (permeabilized cell membrane); and necrotic cells fluoresce red (upper left). Data of Dr. M. Olin, University of Minnesota.

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH II in vitro poly Caspase Apoptosis Detection Reagent

$555

Figure 1. Jurkat cells were treated with staurosporine to induce apoptosis, then stained with FAM- VAD-OPH I (Cat. 6354) or FAM-VAD-FMK FLICA® (Cat. 92), washed and then read on a flow cytometer. Results with the OPH inhibitor (grey solid) are consistent with those achieved using the FMK inhibitor (green outline). FAM-VAD-OPH inhibitors mirror FAM-VAD-FMK analogs for apoptosis detection, and they may be less toxic and more stable. Data courtesy of Dr. M. Olin, University of Minnesota (ICT-202).

ImmunoChemistry Technologies Green Fluorescent FAM-VAD-OPH I in vitro poly Caspase Apoptosis Detection Reagent

$555

Figure 1. Monocytes can secrete active caspase-1, therefore cells were labeled with FAM-FLICA® Caspase-1 (WEHD) (cat. 9161/9162) prior to treatment. Cells were then treated with DMSO (control) or 10 µM nigericin (cat. 6698), stained with Hoechst 33342, fixed (cat. 636), and viewed using EGFP (Ex 470/30, Em 530/50) and DAPI (Ex 375/28, Em 460/50) LED filter cubes at 20X. Increased levels of FAM-FLICA® staining were seen in the nigericin-treated cells. Data courtesy of Dr. Kristi Strandberg, ICT (235:60).

ImmunoChemistry Technologies Green Fluorescent FAM FLICA® Caspase-1 (WEHD) Assay Kit

From $234

Figure 1. The process of autophagy is an intracellular degradation process during which cytosolic organelles and materials are enclosed within an isolation membrane to form an autophagosome. The outer membrane of the autophagosome fuses with the lysosome. The sequestered material is subsequently degraded within the autolysosome.

Figure 2. Cells were either untreated (Black) or treated with 0.5 µM Rapamycin (Orange), 10 µM Chloroquine (Blue), or both 0.5 µM Rapamycin and 10 µM Chloroquine (Red) for 18 hours. After staining with Autophagy Probe - Red Fluorescent, cells were washed and analyzed by flow cytometry (561 nm excitation and a 610/20 emission filter). Treatment with rapamycin or chloroquine increased the fluorescence signal compared to the control. Data courtesy of Dr. Kristi Strandberg (ICT 228:37-40).

ImmunoChemistry Technologies Autophagy Assay - Red Fluorescent

From $199.50

Figure 1. R110-(RR)2 was reconstituted in DMSO, diluted in an aqueous buffer, and then spiked with cathepsin B enzyme and incubated at 37°C for 1 hour. After incubation, fluorescence was analyzed on a Molecular Devices M5e plate reader. The excitation spectrum (black) was generated using an emission of 590 nm. The emission spectrum (green) was generated using an excitation of 460 nm.

Figure 2. Jurkat cells were treated with the cathepsin inhibitors CA-074 Me (blue) and E-64d (gray), or were untreated (green) and then stained with R110-(RR)2. Samples were then analyzed analyzed by flow cytometry (488 nm excitation and a 530/30 emission filter). Treatment with CA-074 Me or E-64d decreased the fluorescence signal detected compared to the untreated control, which displayed a baseline level of cathepsin activity. Data courtesy of Dr. Kristi Strandberg (ICT 231:40-42).

ImmunoChemistry Technologies Green Fluorescent Cathepsin B Assay

From $206

Figure 1. Suspension Jurtkat cells were treated with DMSO (control, A) or 1 µM staurosporine (B) for 4 hours at 37°C, then stained with SR-LETD-FMK (kit cat. 9149/9150) for 1 hour at 37°C. Cells were washed three times and slides prepared. Treated cells appear bright red, indicating a high level of caspase-8 activity (B). Few non-induced cells are red, indicating minimal caspase-8 activity (A, Non-Induced, left). Data courtesy of Mrs. Tracy Murphy, ICT (220:68, 121815).

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-8 (LETD) Assay Kit

From $216

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed in a fluorescence plate reader. The excitation spectrum was generated using an emission of 760 nm. The emission spectrum was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis-inducing agent (right), for 4 hours, then stained with FLICA® 660-DEVD-FMK (cat. 9125) for 1 hour. Cells were washed and analyzed by flow cytometer. The negative control induced caspase-3/7 activity in 4.4% of cells (left); treatment with staurosporine induced caspase-3/7 activity in 70.9% of cells (right). This is a ratio of 16:1. Data courtesy of Mrs. Tracy Murphy, ICT, 13F38, 022013.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-3/7 (DEVD) Assay Kit

$256

ImmunoChemistry Technologies Annexin V-FITC Apoptosis Assay Kit

$267

Figure 2. Flow cytometry analysis of Jurkat cells treated with FLICA® 660-YVAD-FMK. After spiking FLICA® into the cell culture media, the cells were treated with either a negative control (left) or nigericin (20 μM, no.6698) to induce caspase-1 activity (right) for 24 hours. The negative control induced caspase-1 activity in 6.8% of cells (left); treatment with nigericin induced caspase-1 activity in 57.3% of cells (right). This is a ratio of 8:1. Data courtesy of Mrs. Tracy Murphy, ICT, 230-01.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Caspase-1 (YVAD) Assay Kit

$256.50

Figure 1. FLICA® 660 was reconstituted in DMSO, diluted in diH2O, and analyzed on a Molecular Devices Gemini XPS 96-well fluorescence plate reader. The excitation spectrum (grey) was generated using an emission of 760 nm. The emission spectrum (red) was generated using an excitation of 600 nm.

Figure 2. Jurkat cells were treated with a negative control (left) or staurosporine, an apoptosis inducing agent (right), then stained with 660-VAD-FMK (cat. 9120) for 1 hour. Cells were washed twice and read on an Accuri C6 flow cytometer. Treatment with the negative control induced caspase activity in only 8.2% of cells (left), whereas treatment with staurosporine induced caspase activity in 96.5% of cells (right). This is a ratio of 12:1. Data courtesy of Mrs. Tracy Murphy, ICT, 213:48.

ImmunoChemistry Technologies Far-Red Fluorescent FLICA® 660 Poly (active) Caspase (VAD) Assay Kit

$256.50

Figure 1. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

Figure 2. Jurkat cells were treated with DMSO (control) (A) or staurosporine (B), and then dually stained with TMRM and the green FLICA® poly caspase probe FAM-VAD-FMK (cat. 92) and analyzed by flow cytometer. In the staurosporine-treated cells (B), there is loss of orange fluorescence (FL-2) and a concurrent increase in cell-bound green fluorescence (FL-1) due to caspase activation. Treatment with staurosporine induced apoptosis in about 90% of cells (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$290

Figure 1. Jurkat cells were treated with DMSO (negative control) or 1 µM staurosporine to induce apoptosis, then stained with TMRE, and photographed using a photomicroscope using a green excitation filter at 510-560 nm and a 570-620 nm emission filter (left). Healthy cells, containing mitochondria with polarized inner membranes fluoresce bright orange (A) compared with apoptotic cells with depolarized mitochondria(B). The corresponding DIC images are shown (right) (Dr. Brian Lee, ICT).

Figure 2. Jurkat cells were exposed to DMSO as the negative control (dark orange bars) or 50 µM CCCP depolarizing agent (light orange bars) and then incubated with TMRE or TMRM. Aliquots (100 µL) were analyzed in triplicate in a black 96-well plate using a fluorescence plate reader (550 nm excitation and 580 nm emission using a 570 nm cut-off filter). Healthy control cells had a high level of orange fluorescence compared to the CCCP-treated cells. (Ms. Tracy Hanson, ICT).

ImmunoChemistry Technologies TMRE - Orange Fluorescent Mitochondrial Membrane Depolarization Assay

$290

ImmunoChemistry Technologies Green Fluorescent FAM-Phe-DAP Serine Protease Assay Kit

From $212

ImmunoChemistry Technologies Green Fluorescent FAM-Leu-DAP Serine Protease Assay Kit

From $206

ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Leu-CMK Serine Protease Assay Kit

From $206

ImmunoChemistry Technologies Green Fluorescent FAM-Spacer-Phe-CMK Serine Protease Assay Kit

From $206

Figure 1. Jurkat cells, grown in suspension, were incubated with 1 µM staurosporine for 3 hours at 37°C to induce apoptosis, and then labeled with SR-LEHD-FMK (cat. 960/961) for 60 minutes at 37°C. Slides were prepared and imaged using a fluorescence microscope with a broad band pass filter. On slide B, cells appear very bright red, indicating a high level of active caspase-9 in these apoptotic cells. Non-induced cells did not fluoresce (slide A). Data courtesy of Dr. Brian W. Lee, ICT.

Figure 2. Jurkat cells were exposed to two experimental conditions (A and B). Each condition was treated with either DMSO (negative) or staurosporine (apoptotic), then labeled with SR-LEHD-FMK (cat. 960/961). Samples were read in a fluorescence plate reader (550 nm excitation and 590 nm emission using a 570 nm cut-off filter). Staurosporine induced caspase-9 activity 3.3X in both conditions: Condition A (11.0 to 36.5) and Condition B (12.7 to 41.8). Data courtesy of Mrs. Tracy Murphy, ICT 8Y18.

ImmunoChemistry Technologies Red Fluorescent SR-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

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Apoptosis and Autophagy Kits

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