Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional cross-reactive protein additives.
SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Use of this blocking formulation minimizes backgrounds without the use of conventional cross-reactive protein additives. Containing inert non-reactive blockers, SynBlock Blocking Buffer reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. When blocking the coated immunoassay plate wells, SynBlock ELISA Blocking Buffer provides a microhydrated environment to stabilize the coated protein for better binding reactivity during the immunoassay and during long-term storage. SynBlock works on all types of polystyrene plates except Immulon® II microplates. When blocking with SynBlock, ICT recommends Corning® 96-well EIA/RIA Stripwell™ microplates (ICT catalog #25). SynBlock is best used to block coated ELISA plates that require high to very high blocking or that require a protein-free blocking buffer. An antimicrobial agent allows for stable room temperature blocking and long-term refrigerated storage of dried plates prepared with SynBlock. Bulk volumes and custom packaging are available upon request.
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7.4 at 1X
All synthetic materials
Expires two years from date of manufacture
- Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer.
- Incubate 8–24 hours at room temperature (RT).
- Aspirate the coating solution.
- Wash each well twice with ICT’s ELISA Wash Buffer.
- Block the uncoated regions of the ELISA plate by pipetting 300–400 µL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
- Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT.
- Aspirate the blocking buffer; do not wash.
- Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.