FAM-FMK - Caspase 6 Detection by Cell Technology
Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. Cell permeablity permits direct visualization of cytosolic apoptotic events. Apoptotic cell population does not diminish over time. Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure. Works in diverse cell lines: human, rodent, Drosophila. Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells. Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments. Yields both quantitative and qualitative results. Gives strong signal with little background noise.
FAM-FMK - Caspase 6 Detection by Cell Technology
APO LOGIX Carboxyfluoroscein Caspase Detection Kits label active caspases in living cells undergoing apoptosis. Cell Technology’s probes utilize carboxyfluorescein(FAM)-labeled peptide fluoromethyl ketone (FMK) caspase inhibitors (FAM-peptide-FMK). These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems.
Caspase-6
Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscopy
2-8 °C
Ships overnight (domestic), International Priority Shipping
United States
FAM500-1: 25 Tests
Lyophilized FAM Labeled Peptide (FAM-VEID-FMK), 1 vial (Part # 654)
10X Apoptosis Wash Buffer, 15 mL (Part # 3028)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
FAM500-2: 100 Tests
Lyophilized FAM Labeled Peptide (FAM-VEID-FMK), 4 vials (Part # 654)
10X Apoptosis Wash Buffer, 60 mL (Part # 3026)
Fixative, 6 mL (Part # 3027)
Propidium Iodide, 1 mL (Part# 4013)
Product Specific References
| PMID | Publication |
| 16420693 | Jiang, W., et al. 2006. The tumor suppressor DAL-1/4.1B and protein methylation cooperate in inducing apoptosis in MCF-7 breast cancer cells. Mol Cancer, 4. |
| 25054560 | Kovarova, M., et al. 2014. PGE2 Promotes Apoptosis Induced by Cytokine Deprivation through EP3 Receptor and Induces Bim in Mouse Mast Cells. PLoS One. |
| 20943913 | Vohra, B. P., et al. 2010. Amyloid precursor protein cleavage-dependent and -independent axonal degeneration programs share a common nicotinamide mononucleotide adenylyltransferase 1-sensitive pathway. J Neurosci, 13729-38. |