Hoechst 33342 Fluorescent Nucleic Acid Stain

1. Add Hoechst 33342 to the cell sample media at 0.5% v/v. For example, add 1.5 µL Hoechst to 300 µL of cells. 2. Incubate 10-20 minutes at room temperature.
2. Visualize with a fluorescence microscope by using a UV excitation and blue emission filter. The blue Hoechst stain fluoresces at 461 nm.
3. Alternatively, cells may be analyzed with a flow cytometer using a UV excitation source.
4. When bound to dsDNA, the maximum absorption is 350 nm and the maximum emission is 461.

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