The MitoPT JC-1 Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, JC-1 reagent is dispersed through the cell and will fluoresce green. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.

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MitoPT JC-1 Assay
SKU: 924

Size: 100 Tests
Sale price$219.00

Bulk Order MitoPT JC-1 Assay

Loss of mitochondrial membrane potential is indicative of apoptosis. ICT’s MitoPT JC-1 Assay utilizes the potentiometric fluorescent dye JC-1 to detect mitochondrial membrane permeability and membrane depolarization. The JC-1 dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT JC-1 becomes distributed throughout the cytosol. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
Mitochondrial depolarization
488 nm / 590 nm & 527 nm
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples.
  2. Create controls with CCCP.
  3. Dilute 10X Assay Buffer 1:10 with diH2O.
  4. Reconstitute MitoPT JC-1 with DMSO.
  5. Dilute MitoPT JC-1 with 1X Assay Buffer.
  6. Add MitoPT JC-1 to each sample.
  7. Incubate 15-30 minutes.
  8. Wash cells: add 1X Assay Buffer and spin cells (twice).
  9. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.
Kit 911: 400 Tests
  • MitoPT JC-1 Reagent, 400 Tests, #6260
  • 10X Assay Buffer (2 x 125 mL), #6259
  • CCCP, 50 mM, 600 µL, #6258
  • Kit Manual
  • Kit 924: 100 Tests
  • MitoPT JC-1 Reagent, 100 Tests, #6261
  • 10X Assay Buffer, 60 mL, #685
  • CCCP, 50 mM, 125 µL, #6257
  • Kit Manual
  • Product Specific References

    PMID Publication
    37504440Bingol Ozakpinar, O., et al. 2023. Carbon Nanofiber-Sodium Alginate Composite Aerogels Loaded with Vitamin D: The Cytotoxic and Apoptotic Effects on Colon Cancer Cells. Gels (Basel, Switzerland).
    35508969Kraemer, B.F., et al. 2022. Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia. Molecular medicine (Cambridge, Mass.), 51.
    35965114Zhong, G., et al. 2022. Blood-brain barrier Permeable nanoparticles for Alzheimer's disease treatment by selective mitophagy of microglia. Biomaterials, 121690.
    35947192Yoshioka, H., et al. 2022. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone and mineral metabolism.
    36262666Funakoshi, A., et al. 2022. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations, 100153.
    36331786Bülbül, B., et al. 2022. Novel 1,2,4-triazoles derived from Ibuprofen: synthesis and in vitro evaluation of their mPGES-1 inhibitory and antiproliferative activity. Molecular diversity.
    34193088Wu, W., et al. 2021. BCL-xL is Correlated With Disease Severity in Neonatal Infants With Sepsis. Research Square, 295.

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