MitoPT JC-1 Assay
The MitoPT JC-1 Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye JC-1. When accumulated in a negatively charged polarized mitochondria, JC-1 fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, JC-1 reagent is dispersed through the cell and will fluoresce green. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
MitoPT JC-1 Assay
Loss of mitochondrial membrane potential is indicative of apoptosis. ICT’s MitoPT JC-1 Assay utilizes the potentiometric fluorescent dye JC-1 to detect mitochondrial membrane permeability and membrane depolarization.
The JC-1 dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The JC-1 dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT JC-1 becomes distributed throughout the cytosol. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
JC-1
Mitochondrial depolarization
488 nm / 590 nm & 527 nm
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
Cell culture
-20°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
- Prepare samples.
- Create controls with CCCP.
- Dilute 10X Assay Buffer 1:10 with diH2O.
- Reconstitute MitoPT JC-1 with DMSO.
- Dilute MitoPT JC-1 with 1X Assay Buffer.
- Add MitoPT JC-1 to each sample.
- Incubate 15-30 minutes.
- Wash cells: add 1X Assay Buffer and spin cells (twice).
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. MitoPT JC-1 excites at 488 nm. Aggregated MitoPT JC-1 (orange) emits at 590 nm, monomeric MitoPT JC-1 (green) emits at 527 nm.
Kit 911: 400 Tests
MitoPT JC-1 Reagent, 400 Tests, #6260
10X Assay Buffer (2 x 125 mL), #6259
CCCP, 50 mM, 600 µL, #6258
Kit Manual
Kit 924: 100 Tests
MitoPT JC-1 Reagent, 100 Tests, #6261
10X Assay Buffer, 60 mL, #685
CCCP, 50 mM, 125 µL, #6257
Kit Manual
Product Specific References
| PMID | Publication |
| 37504440 | Bingol Ozakpinar, O., et al. 2023. Carbon Nanofiber-Sodium Alginate Composite Aerogels Loaded with Vitamin D: The Cytotoxic and Apoptotic Effects on Colon Cancer Cells. Gels (Basel, Switzerland). |
| 35508969 | Kraemer, B.F., et al. 2022. Platelet mitochondrial membrane depolarization reflects disease severity in patients with preeclampsia. Molecular medicine (Cambridge, Mass.), 51. |
| 35965114 | Zhong, G., et al. 2022. Blood-brain barrier Permeable nanoparticles for Alzheimer's disease treatment by selective mitophagy of microglia. Biomaterials, 121690. |
| 35947192 | Yoshioka, H., et al. 2022. Deletion of Tfam in Prx1-Cre expressing limb mesenchyme results in spontaneous bone fractures. Journal of bone and mineral metabolism. |
| 36262666 | Funakoshi, A., et al. 2022. Cholecystokinin receptor antagonist suppresses melanoma growth by inducing apoptosis of tumor cells. JID Innovations, 100153. |
| 36331786 | Bülbül, B., et al. 2022. Novel 1,2,4-triazoles derived from Ibuprofen: synthesis and in vitro evaluation of their mPGES-1 inhibitory and antiproliferative activity. Molecular diversity. |
| 34193088 | Wu, W., et al. 2021. BCL-xL is Correlated With Disease Severity in Neonatal Infants With Sepsis. Research Square, 295. |