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Cell Viability Assays

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Fixative
Figure 1. Jurkat cells were stained with DAF-2DA dye (Kit 9155), washed, and then treated with DMSO control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram). Cells were read on the FL1 channel of a flow cytometer. The median fluorescence intensity (MFI) of DEA NONOate treated cells was 3.5-fold higher than for the control cells. Data also overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76.

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