Propidium Iodide Stain
- Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature.
- Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm.
- Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3.
- When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.