Propidium Iodide Stain
- Add PI to the cell sample media at 0.5% v/v. For example, add 1.5 µL PI to 300 µL of cells. 2. Incubate 5-10 minutes at room temperature.
- Visualize with a fluorescence microscope with excitation at 488 nm and emission at 635 nm.
- Alternatively, cells can be analyzed with a flow cytometer using a blue laser at 488 nm or a green laser at 525 nm. Read fluorescent output in FL-2 or FL-3.
- When bound to DNA, the maximum absorption is 535 nm and the maximum emission is 617.
Wu, R;Ohara, RA;Jo, S;Liu, TT;Ferris, ST;Ou, F;Kim, S;Theisen, DJ;Anderson, DA;Wong, BW;Gershon, T;Schreiber, RD;Murphy, TL;Murphy, KM. Mechanisms of CD40-dependent cDC1 licensing beyond costimulation. Nature Immunology. 2022 October 21; doi: 10.1038/s41590-022-01324-w. Full Abstract
Ventura, PMO;Gakovic, M;Fischer, BA;Spinelli, L;Rota, G;Pathak, S;Khameneh, HJ;Zenobi, A;Thomson, S;Birchmeier, W;Cantrell, DA;Guarda, G. Concomitant deletion of Ptpn6 and Ptpn11 in T cells fails to improve anticancer responses. EMBO reports. 2022 October 4; doi: 10.15252/embr.202255399. Abstract
Shu, L;Du, C. PHLDA1 promotes sevoflurane-induced pyroptosis of neuronal cells in developing rats through TRAF6-mediated activation of Rac1. Neurotoxicology. 2022 September 22; doi: 10.1016/j.neuro.2022.09.007. Article
Tian, J;Zhou, D;Xiang, L;Xie, B;Wang, B;Li, Y;Liu, X. Calycosin represses AIM2 inflammasome-mediated inflammation and pyroptosis to attenuate monosodium urate-induced gouty arthritis through NF-κB and p62-Keap1 pathways. Drug Development Research. 2022 September 7; doi: 10.1002/ddr.21985. Text
LaRock, DL;Johnson, AF;Wilde, S;Sands, JS;Monteiro, MP;LaRock, CN. Group A Streptococcus induces GSDMA-dependent pyroptosis in keratinocytes. Nature. 2022 May 1; doi: 10.1038/s41586-022-04717-x. Full Article