Goat Anti-Human IgG recognizes human IgG F(c) fragment. This HRP-conjugated secondary antibody was purified using antigen affinity chromatography and is suitable for ELISA and Western blotting techniques. The IgG component of this conjugate is specific for normal human IgG and is non-reactive with k or l light chains. The IgG-HRP conjugate contains between 2 and 4 HRP molecules per IgG molecule.
ICT’s HRP Goat Anti Human IgG Fc is suitable for ELISA, Western blotting, and histology techniques. Goats were immunized with a purified preparation of human IgG Fc fragment that was obtained from a purified normal human IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled human IgG Fc protein. Affinity purified, anti-human IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-human IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule. Using ID, IEP, and ELISA analysis techniques, the IgG component of this conjugate was specific for normal human IgG and was non-reactive with k or l light chains. This conjugate is suitable for ELISA and Western blotting techniques. The conjugate is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent.
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