Goat Anti-Mouse IgG Fc recognizes mouse IgG F(c) fragment. This HRP-conjugated antibody was purified using antigen affinity techniques and is suitable for ELISA and Western blotting procedures. The IgG component of this antibody conjugate is specific for normal mouse IgG and is non-reactive with IgM or IgE serum proteins. The IgG-HRP conjugate contains between 2 and 4 HRP molecules per IgG molecule.
ICT’s HRP Goat Anti Mouse IgG Fc is suitable for ELISA, Western blotting, and histology techniques.
Goats were immunized with a purified preparation of mouse IgG Fc fragment that was obtained from a purified normal mouse IgG antibody protein pool. The hyperimmune IgG fraction was initially pre-adsorbed over solid phase gels bearing covalently coupled mouse Fab protein. Subsequently, the goat anti-Mouse IgG Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled mouse IgG Fc protein.
Affinity purified, anti-mouse IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-mouse IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process produces a Goat IgG HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule.
Using ID and IEP analysis techniques, the IgG component of this Goat anti-Mouse IgG Fc HRP conjugate was specific for normal mouse IgG and was non-reactive with IgM or IgE serum proteins.
The conjugate is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent.