Goat anti-Rabbit IgG recognizes rabbit IgG F(c) fragment. This HRP-conjugated secondary antibody was purified using antigen affinity techniques and is suitable for ELISA and Western blotting procedures. The IgG component of this HRP conjugate is specific for normal rabbit IgG and is non-reactive with other serum proteins. The IgG-HRP conjugate contains between 2 and 4 HRP molecules per IgG molecule.
ICT’s Goat Anti Rabbit IgG Fc HRP Conjugated Antibody is suitable for ELISA and Western blotting analysis techniques. Goats were immunized with a purified preparation of rabbit IgG Fc fragment that was obtained from a purified normal rabbit IgG antibody protein pool. The hyperimmune IgG fraction was initially solid phase adsorbed to obtain class specificity. Subsequently, the anti-Fc antibody was adsorbed to and eluted from a second solid phase immunoaffinity purification gel containing covalently coupled rabbit IgG Fc protein. Affinity purified, anti-rabbit IgG Fc specific goat IgG was covalently coupled with HRP using a maleimide (M)- facilitated conjugation technique. In this process, free sulfhydryl groups are added to the anti-rabbit IgG Fc prep just prior to its reaction with a 4-fold molar excess of HRP-M. This process leads to an IgG-HRP conjugate that contains between 2 and 4 HRP molecules per IgG molecule. Using ELISA and IEP analysis techniques, the IgG component of this conjugate was specific for normal rabbit serum IgG and was non-reactive with other serum proteins. Goat Anti Rabbit IgG Fc HRP Conjugated Antibody is dissolved in a Tris based conjugate stabilization buffer containing 50 ppm ProClin 300 anti-microbial agent.
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