Preserve activity of HRP conjugates and reduce background signal. Neptune™ HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques. This product is best for immunoassays using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich ELISA formats.
- Protect the HRP conjugate stabilizer from light. This may be done by covering the container with aluminum foil.
- Add the HRP conjugate at the target concentration to Neptune HRP Conjugate Stabilizer. For example, if diluting the HRP conjugate 1:5000, add 10 µL conjugate stock to 49.99 mL Neptune HRP Conjugate Stabilizer.
- Mix for 15 minutes.
- Use the diluted HRP conjugate in the immunoassay.
- Store remaining HRP conjugate solution at 2-8°C. Protect from direct exposure to light.