Assay Diluents are additive components used in an ELISA that equalize any differences between the sample matrices (serum, plasma, urine, cell culture fluid) and the calibrator diluent used to generate the standard curve of the ELISA. ICT’s Assay Diluent Optimization Pack, containing four 100 mL ELISA development buffers (General Assay Diluent, IgM-Reducing Assay Diluent, Neptune Assay Diluent, and Antigen-Down Assay Diluent), offers an economical method of addressing matrix effects and optimizing signal-to-noise ratio in ELISA development projects.
- To quickly determine which Assay Diluent formulation is best for the assay, all four Assay Diluents can be run in parallel on the same IgG-coated ELISA plate.
- Determine the initial dynamic analyte detection range of the ELISA curves that were prepared in each of the four different Assay Diluent types.
- Make test spikes into serum samples.
- Read the spiked serum sample wells from each of the four AD-associated standard curves to see which Assay Diluent gives the best approximation of the spike concentration that was added to the serum samples.
- In cases where a high level of matrix complexity interference is not detected, an additional Assay Diluent may not be required to obtain a useful standard curve and derived sample analysis.