SR-FLICA® Poly Caspase Assay Kit
Detect active caspase enzymes with the SR FLICA® Poly Caspase Assay Kit. This in vitro assay employs the fluorescent inhibitor probe SR-VAD-FMK to label active caspase enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry.
SR-FLICA® Poly Caspase Assay Kit
Caspases play important roles in apoptosis and inflammation. ICT’s FLICA poly caspase assay kit is used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues.
SR-VAD-FMK
Poly caspases
550-580 nm / 590-600 nm
Flow Cytometer, Fluorescence Plate Reader, Fluorescence Microscope
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
- Prepare samples and controls.
- Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
- Reconstitute FLICA with 50 μL DMSO.
- Dilute FLICA 1:5 by adding 200 μL PBS.
- Add diluted FLICA to each sample at 1:30 – 1:60. For example, to stain at 1:30, add 10 μL to 290 μL of cultured cells. To stain at 1:60, add 5 μL to 295 μL of cultured cells.
- Incubate approximately 1 hour.
- Remove media and wash cells 3 times: add 1X Apoptosis Wash Buff er and spin cells.
- If desired, label with additional stains, such as Hoechst, 7-AAD, or an antibody.
- If desired, fix or embed cells.
- Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. SR-FLICA excites at 550-580 nm and emits at 590-600 nm.
Kit 916: 25 Tests
FLICA Poly Caspase Reagent (SR-VAD-FMK), 1 vial, #679
10X Apoptosis Wash Buffer, 15 mL, #635
Fixative, 6 mL, #636
Hoechst 33342, 1 mL, #639
Kit Manual
Kit 917: 100 Tests
FLICA Poly Caspase Reagent (SR-VAD-FMK), 4 vials, #679
10X Apoptosis Wash Buffer, 60 mL, #634
Fixative, 6 mL, #636
Hoechst 33342, 1 mL, #639
Kit Manual
Product Specific References
| PMID | Publication |
| 37409377 | Wang, H., et al. 2023. Adverse effects of knocking down chitin synthase A on female reproduction in Culex pipiens pallens (Diptera: Culicidae). Pest management science. |
| 36271147 | Wu, R., et al. 2022. Mechanisms of CD40-dependent cDC1 licensing beyond costimulation. Nature immunology. |