Fluorescent NADP/NADPH Detection Kit by Cell Technology
Detection of NADP/NADPH content in cells or tissue extracts. Detection of NADP/NADPH levels in antioxidation and oxidative stress. Detection of NADP/NADPH levels in cell death, energy metabolism, and mitochondria function. Species Independent – NADP/NADPH detection in Bacterial, fungal and plant cells. Highly Sensitive – Detects up to 4nM NADP and NADPH. Highly Specific – No Cross reactivity with NAD/NADH.
Fluorescent NADP/NADPH Detection Kit by Cell Technology
The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is reduced in
the presence of NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH
via an enzyme-coupled reaction. The enzymes in the reactions specifically react with NADP/NADPH and
not with NAD/NADH.
Fluorescence plate reader
Store contents as labeled
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NADPH100-2: 100 Tests
Part # 6022: Enzyme Mix, 170μL x 1 (2-8C)
Part # 3050: NADP/NADPH Lysis Solution, 12.5mL x 1 (2-8C)
Part # 3057 (Green label): NADP Extraction Buffer, 22 mL x 1 (2-8C)
Part # 3058 (Magenta label): NADPH Extraction Buffer, 22 mL x 1 (2-8C)
Part # 3049: Reaction Buffer, 11 mL x 1 (2-8C)
Part # 3052: Standard curve diluent, 15 mL x 1 (2-8C)
Part # 4019: NADPH Detection reagent, 110 μL x 1 (-20C)
Part # 3048: 3X Substrate Mix, 6mL x 1 (-20C)
Part # 7014: NADPH Standard, 3 vials dried (-20C)
NADPH100-3: 500 TestsPart # 6022: Enzyme Mix, 170μL x 5 (2-8C)
Part # 3050: NADP/NADPH Lysis Solution, 12.5mL x 5 (2-8C)
Part # 3057 (Green label): NADP Extraction Buffer, 22 mL x 5 (2-8C)
Part # 3058 (Magenta label): NADPH Extraction Buffer, 22 mL x 5 (2-8C)
Part # 3049: Reaction Buffer, 11 mL x 5 (2-8C)
Part # 3052: Standard curve diluent, 15 mL x 5 (2-8C)
Part # 4019: NADPH Detection reagent, 110 μL x 5 (-20C)
Part # 3048: 3X Substrate Mix, 6mL x 5 (-20C)
Part # 7014: NADPH Standard, 3 vials dried (-20C)
NADPH100-3: 500 Tests