Cell Technology's Total Cholesterol kit is a simple one step assay which can be used either as a Fluorimetric or Colorimetric assay to help detect the level of total cholesterol in samples. Used to study the effect of drugs on Cholesterol Metabolism. Highly Sensitive – Detects up to 200nM of Cholesterol.
Cholesterol is a lipid present in the cell membranes of eukaryotes and circulates in the blood stream. It is
used in the biosynthesis of hormones, and plays an important role in cell signaling processes. Cholesterol
exists as a free acid, as well as, in the esterified form as cholesteryl esters. Elevated levels of cholesterol
are indicated in atherosclerosis and heart disease, and are the subject of large amount of research focused
on cholesterol metabolism. Fluoro CholesterolTM – Total Cholesterol Detection Kit provides a fluorimetric
or colorimetric method for determination of total cholesterol in serum and plasma samples. The assay is
based on an enzyme-coupled reaction that detects both free cholesterol and cholesterol esters.
Absorbance or Fluorescence plate reader
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