pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)


pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

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pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets (SUBP)
SKU: 6279

Volume: 100 mL
Price:
Sale price$45
pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays. Alkaline phosphatase substrates are used in ELISA assays to detect alkaline phosphatase-conjugated molecules via a colorimetric enzymatic reaction. pNPP 1-Component AP Microwell Substrate with Stabilizing Pellets contains para-Nitrophenylphosphate (pNPP). This formulation of pNPP substrate also contains a stabilizer to extend the shelf life of the alkaline phosphatase substrate on your benchtop to 2-3 days. The alkaline phosphatase substrate reaction may be stopped with the use of a stop solution, Stop Solution for pNPP AP Microwell Substrates. Bulk volumes are available upon request.
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
9.6-10.2
1X
United States
  1. Run ELISA according to the specific protocol through the conjugate incubation step.
  2. Wash the wells three or four times with 1X ELISA Wash Buffer (catalog #652) to remove any residual AP-conjugate.
  3. Bring SUBP to room temperature; protect from light.
  4. Pipette 100 µL SUBP into each well of the plate.
  5. Incubate SUBP 10-60 minutes. Monitor the color intensity.
  6. Read the plate between 405-420 nm and analyze. Alternatively, stop the reaction by adding 100 µL/well STOPP (catalog #6284) and read the samples between 405-420 nm within 1 hour.

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