aCella - AChE by Cell Technology

from ImmunoChemistry Technologies Datasheet Safety Data Sheet Protocol
1 Citation

Safe – Non Radioactive Enzyme activity assay. Versatile: Nerve Gas, Pesticide Monitoring, drug screening Applications Homogenous – One-step, no wash assay. FAST – Results in 30 secs – 5 minutes. Highly Sensitive HTS – Adaptable for High Throughput format

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aCella - AChE by Cell Technology
Tacrine (a mixed-mode inhibitor of AChE) was serially diluted in DI water. Next 10mL of the diluted Tacrine (x axis labeling represents mM final concentration of Tacrine) was added to a white opaque 96-well microplate along with 50 mL of component A (AChE enzyme). The samples were incubated for 5 minutes after which 50mL of component B was added to all the wells. Data were collected using a luminometer. Data shown represents T=2 minutes after the addition of component B.
Malathion was first diluted in DMSO and subsequently serially diluted in Di water. 10μL of the diluted Malathion (x axis represents μM final concentration of Malathion) was added to a white opaque 96-well microplate followed by 50 μL of component A (AChE enzyme). The mixture was incubated for 15 minutes, after which 50 μL of component B was added to all the wells. Data were collected using a luminometer. Data shown is at T= 5 minutes. Data shown represents T=2.5 minutes after the addition of component B.
aCella - AChE by Cell Technology
aCella - AChE by Cell Technology
Tacrine (a mixed-mode inhibitor of AChE) was serially diluted in DI water. Next 10mL of the diluted Tacrine (x axis labeling represents mM final concentration of Tacrine) was added to a white opaque 96-well microplate along with 50 mL of component A (AChE enzyme). The samples were incubated for 5 minutes after which 50mL of component B was added to all the wells. Data were collected using a luminometer. Data shown represents T=2 minutes after the addition of component B.
Malathion was first diluted in DMSO and subsequently serially diluted in Di water. 10μL of the diluted Malathion (x axis represents μM final concentration of Malathion) was added to a white opaque 96-well microplate followed by 50 μL of component A (AChE enzyme). The mixture was incubated for 15 minutes, after which 50 μL of component B was added to all the wells. Data were collected using a luminometer. Data shown is at T= 5 minutes. Data shown represents T=2.5 minutes after the addition of component B.

aCella - AChE by Cell Technology
SKU: CLACHE100-2

Size: 100 Tests
Price:
$435.00

Bulk Order aCella - AChE by Cell Technology

A highly sensitive, rapid and simple bioluminescence assay for monitoring acetylcholinesterase. The kit uses the natural substrate, acetylcholine. A series of coupled enzyme reactions quickly translates the presence of active AChE into a change in the luminance of the reaction.
Luminometer
-20°C
Ships overnight (domestic), International Priority Shipping
United States
CLACHE100-2: 100 Tests
  • 1. Component A: Contains Acetylcholinesterase Part# 3023 x 1
  • 2. Component B: Contains Detection reagent, acetylcholine and coupled enzyme reaction Part# 3024 x 1
  • 3. Component C: Control to measure maximum Luminescence Part# 3025 x 1

    • CLACHE100-3: 500 Tests:
  • 1. Component A: Contains Acetylcholinesterase Part# 3023 x 5
  • 2. Component B: Contains Detection reagent, acetylcholine and coupled enzyme reaction Part# 3024 x 5
  • 3. Component C: Control to measure maximum Luminescence Part# 3025 x 5

    • CLACHE100-4: 1000 Tests
  • 1. Component A: Contains Acetylcholinesterase Part# 3023 x 10
  • 2. Component B: Contains Detection reagent, acetylcholine and coupled enzyme reaction Part# 3024 x 10
  • 3. Component C: Control to measure maximum Luminescence Part# 3025 x 10

    • Product Specific References

    PMID Publication
    23832808Marques, S.M., et al. 2014. Quantitative analysis of organophosphorus pesticides in freshwater using an optimized firefly luciferase‐based coupled bioluminescent assay. Luminescence, 378-385.

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