aCella - Tox - an assay designed to measure cell cytoxicity

A bioluminescence, non-radioactive cytotoxicity assay designed to quantitatively measure the release of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from mammalian cell lines or bacterial cells.



SKU: CLATOX100-3

Size: 500 Tests
Price:
Sale price$883.00
GAPDH is an important enzyme in the glycolysis and gluconeogenesis pathways. This homotetrameric enzyme catalyzes the oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate in the presence of cofactor and inorganic phosphate. In the aCella-TOX reaction scheme the release of GAPDH is coupled to the activity of the enzyme 3-Phosphoglyceric Phosphokinase (PGK) to produce ATP. ATP is detected via the luciferase, luciferin Bioluminescence methodology. Further, aCella-TOX is a homogeneous cytotoxicity assay; alternatively in dual mode, aCella-TOX can measure cytotoxicity and cell viability in the same plate. Culture supernatants can also be removed from the original plate and assayed in a different plate, allowing kinetics runs to be set up. The assay is non-destructive, allowing the monitoring of additional parameters such as gene expression.
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CLATOX100-3P: 500 Tests (with 5 Lumi Plates + 5 TC Plates)
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)
  • 7. 5 Luminescence Plates + 5 Tissue Culture Plates (Catalog# CLATOX100-3P)

  • CLATOX100-3: 500 Tests
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)

  • CLATOX100-4: 1000 Tests
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 2 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 2 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 2 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 2 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 2 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 2 (Storage: 2-8°C)

  • CLATOX100-3L: 500 Tests (with 5 Lumi Plates)
  • 1. 4X Enzyme Assay Reagent Part# 6001 x 1 (Storage: -20°C)
  • 2. Enzyme Assay Diluent Part# 3008 x 1 (Storage: 2-8°C)
  • 3. 50X Detection Reagent Part# 6002 x 1 (Storage: -20°C)
  • 4. 5.5X Detection Assay Diluent Part# 3009 x 1 (Storage: -20°C)
  • 5. Glyeraldehyde 3-Phosphate-G3P Part# 6003 x 1 (Storage: -20°C)
  • 6. Lysis Buffer Part# 3035 x 1 (Storage: 2-8°C)
  • 7. 5 Luminescence Plates (Catalog# CLATOX100-3L)
  • Product Specific References

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    36211807Guo, L., et al. 2022. A newly discovered PD-L1 B-cell epitope peptide vaccine (PDL1-Vaxx) exhibits potent immune responses and effective anti-tumor immunity in multiple syngeneic mice models and (synergizes) in combination with a dual HER-2 B-cell vaccine (B-Vaxx). Oncoimmunology, 2127691.
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    28970093Hoffman, F., et al. 2018. A mathematical model of antibody-dependent cellular cytotoxicity (ADCC). Journal of Theoretical Biology, 39-50.
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