A homogenous platform that utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The assay can be used for high throughput screening.
Cell Technology's APO 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm. As a result of a novel and proprietary Lysis Buffer System, the APO 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.
Caspase-3/7
Fluorescence Plate Reader
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APO200-2: 100 Tests
Part# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 1 Vial (-20C)
Part# 3005: Cell Lysis Buffer, 1 Bottle (2-8C)
APO200-3: 500 TestsPart# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 5 Vials (-20C)
Part# 3005: Cell Lysis Buffer, 5 Bottles (2-8C)
APO200-4: 1000 TestsPart# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 10 Vials (-20C)
Part# 3005: Cell Lysis Buffer, 10 Bottles (2-8C)
APO200-3: 500 Tests
APO200-4: 1000 Tests
Product Specific References
PMID | Publication |
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24960609 | Tourette, C., et al. 2014. The Wnt receptor Ryk reduces neuronal and cell survival capacity by repressing FOXO activity during the early phases of mutant huntingtin pathogenicity. PLoS Biol, e1001895. |
24407293 | Tourette, C., et al. 2014. A Large Scale Huntingtin Protein Interaction Network Implicates Rho GTPase Signaling Pathways in Huntington Disease. J Biol Chem, 6709-6726. |
26232724 | Kim, H. J., et al. 2015. Intracellular transduction of TAT-Hsp27 fusion protein enhancing cell survival and regeneration capacity of cardiac stem cells in acute myocardial infarction. J Control Release, 55-72. |
24333653 | Castri, P., et al. 2015. Poly(ADP-ribose) polymerase-1 and its cleavage products differentially modulate cellular protection through NF-kB-dependent signaling. Biochim Biophys Acta, 640-51. |
22709585 | Siddiqui, A., et al. 2012. Mitochondrial DNA damage Is associated with reduced mitochondrial bioenergetics in Huntington's disease. Free Radic Biol Med, 1478-88. |