A homogenous platform that utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The assay can be used for high throughput screening.
Cell Technology's APO 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm. As a result of a novel and proprietary Lysis Buffer System, the APO 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.
Caspase-3/7
Fluorescence Plate Reader
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APO200-2: 100 Tests
Part# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 1 Vial (-20C)
Part# 3005: Cell Lysis Buffer, 1 Bottle (2-8C)
APO200-3: 500 TestsPart# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 5 Vials (-20C)
Part# 3005: Cell Lysis Buffer, 5 Bottles (2-8C)
APO200-4: 1000 TestsPart# 4005: Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 10 Vials (-20C)
Part# 3005: Cell Lysis Buffer, 10 Bottles (2-8C)
APO200-3: 500 Tests
APO200-4: 1000 Tests