Apo Active PE - Caspase 3 by Cell Technology

from ImmunoChemistry Technologies Datasheet Safety Data Sheet
1 Citation

Antibody Specific Active Caspase 3 Phycoerythrin (PE) Detection Kit is highly specific for active human and murine. The kit utilizes a rabbit affinity purified polyclonal antibody against a 163-175.

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Apo Active PE - Caspase 3 by Cell Technology
Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Apo Active PE - Caspase 3 by Cell Technology
Apo Active PE - Caspase 3 by Cell Technology
Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

Apo Active PE - Caspase 3 by Cell Technology
SKU: PAB200-2

Size: 100 Tests
Price:
$490.00

Bulk Order Apo Active PE - Caspase 3 by Cell Technology

Cell Technology's APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3. This neo epitope is present on the p18 subunit of cleaved caspase 3. Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.
Caspase-3/7
Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader
2-8°C
Ships overnight (domestic), International Priority Shipping
United States
  • Part# 1002: Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
  • Part# 2004: PE Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
  • Part# 3001: 10X Fixative (Room Temperature)

    • Product Specific References

    PMID Publication
    24463278Ueno, S., et al. 2014. Aberrant expression of SALL4 in acute B cell lymphoblastic leukemia: Mechanism, function, and implication for a potential novel therapeutic target. Exp Hematol, 307-316.

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