Eosinophil Peroxidase Detection Kit by Cell Technology

from ImmunoChemistry Technologies Datasheet Safety Data Sheet Protocol

Can monitor multiple time points to follow kinetics. One-step, no wash assay. Adaptable for High Throughput format. Highly Sensitive.

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Eosinophil Peroxidase Detection Kit by Cell Technology
Eosinophil peroxidase was titrated in 1X reaction buffer and 50uL of each point was added in triplicate to 96 black wells. 50uL of reaction cocktail was added and the reaction was incubated in the dark at room temperature for 30 minutes. Fluorescence was read at 530nm excitation and emission detected at 590nm.
Eosinophil Peroxidase Detection Kit by Cell Technology
Eosinophil Peroxidase Detection Kit by Cell Technology
Eosinophil peroxidase was titrated in 1X reaction buffer and 50uL of each point was added in triplicate to 96 black wells. 50uL of reaction cocktail was added and the reaction was incubated in the dark at room temperature for 30 minutes. Fluorescence was read at 530nm excitation and emission detected at 590nm.

Eosinophil Peroxidase Detection Kit by Cell Technology
SKU: FLEPO100-2

Size: 100 Tests
Price:
$460.00

Bulk Order Eosinophil Peroxidase Detection Kit by Cell Technology

Eosinophil peroxidase (EPO) is the most abundant enzyme found in eosinophil and is the major cytotoxic agent released by activated eosinophils. It uses hydrogen peroxide to generate reactive oxidants from halides and pseudo halide thiocyanate and has been shown to have antimycobacterial activity. It is implicated in tissue damage that occurs in asthma and other diseases. Eosinophils are involved in the initiation and propagation of diverse inflammatory responses as well as modulators of innate and adaptive immunity.
Fluorescence plate reader
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  • Part# 4016: Detection Reagent, 1 Vial (-20C)
  • Part# 3002: 10X Assay Buffer, 60mL (2-8C)
  • Part# 3012: Hydrogen Peroxide, 1000µL of a Stabilized Solution (2-8C)
  • Part# 6016: Eosinophil Peroxidase, 1 Vial (100µL at 10Units/mL) (2-8C)

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