Detect active caspase enzymes with the FAM-FLICA Poly Caspase Assay Kit. This in vitro assay employs the fluorescent inhibitor probe FAM-VAD-FMK to label active caspase enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry

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FAM FLICA Poly Caspase Assay Kit
SKU: 91

Size: 25 Tests
Price:
Sale price$201
Caspases play important roles in apoptosis and inflammation. ICT’s FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Poly Caspase probe allows researchers to assess caspase activation.
FAM-VAD-FMK
Poly Caspases
492 nm / 520 nm
Flow Cytometer, Fluorescence Plate Reader, Fluorescence Microscope
Cell culture
2-8°C
Domestic: Overnight Delivery; International: Priority Shipping
United States
  1. Prepare samples and controls
  2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20.
  3. Reconstitute FLICA with 50 µL DMSO.
  4. Dilute FLICA 1:5 by adding 200 µL PBS.
  5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells).
  6. Incubate approximately 1 hour.
  7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells.
  8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody.
  9. If desired, fix cells.
  10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm.

If working with adherent cells, please see the manual for additional protocols.

Kit 91: 25 Tests
  • FLICA Poly Caspase Reagent (FAM-VAD-FMK), 1 vial, #637
  • 10X Apoptosis Wash Buffer, 15 mL, #635
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Kit 92: 100 Tests
  • FLICA Poly Caspase Reagent (FAM-VAD-FMK), 4 vials, #637
  • 10X Apoptosis Wash Buffer, 60 mL, #634
  • Fixative, 6 mL, #636
  • Propidium Iodide, 1 mL, #638
  • Hoechst 33342, 1 mL, #639
  • Kit Manual
  • Gioulbasani M, Galaras A, Grammenoudi S, Moulos P, Dent AL, Sigvardsson M, Hatzis P, Kee BL, Verykokakis M. The transcription factor BCL-6 controls early development of innate-like T cells. Nat Immunol. 2020 Sep;21(9):1058-1069. doi: 10.1038/s41590-020-0737-y. Epub 2020 Jul 27. Abstract

    Dodard G, Tata A, Erick TK, Jaime D, Miah SMS, Quatrini L, Escalière B, Ugolini S, Vivier E, Brossay L. Inflammation-Induced Lactate Leads to Rapid Loss of Hepatic Tissue-Resident NK Cells. Cell Rep. 2020 Jul 7;32(1):107855. doi: 10.1016/j.celrep.2020.107855. Full Text

    Wandel MP, Kim BH, Park ES, Boyle KB, Nayak K, Lagrange B, Herod A, Henry T, Zilbauer M, Rohde J, MacMicking JD, Randow F. Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Nat Immunol. 2020 Aug;21(8):880-891. doi: 10.1038/s41590-020-0697-2. Epub 2020 Jun 15. Abstract

    Lin JX, Du N, Li P, Kazemian M, Gebregiorgis T, Spolski R, Leonard WJ. Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells. Nat Commun. 2017. Nov 6;8(1):1320. doi: 10.1038/s41467-017-01477-5. Full Text

    Chen YL, Xu G, Liang X, Wei J, Luo J, Chen GN, Yan XD, Wen XP, Zhong M, Lv X. Inhibition of hepatic cells pyroptosis attenuates CLP-induced acute liver injury. Am J Transl Res. 2016. Full text.

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